Mouse anti-Human CD154, soluble Monoclonal Antibody | anti-CD40LG antibody

CD154, soluble (CD40 Ligand, CD40-L, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Superfamily Member 5, TNFSF5)

Cat. Num. AAA609808

• Gene Names: CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CD154; soluble; Monoclonal Antibody; soluble (CD40 Ligand; CD40-L; CD40L; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; T-cell Antigen GP39; TNF-related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; TNF Ligand Superfamily Member 5; TNFSF5); Anti -CD154; anti-CD40LG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9A159
• Specificity: Recognizes human CD154, a 32kD glycoprotein also known as CD40 ligand. It is a member of the TNF family of molecules.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH7.2, 0.09% sodium azide.

• Applicable Applications for anti-CD40LG antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Recombinant human soluble CD154 conjugated to BSA.
• Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the myeloma Sp2/0 cell line.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CD40LG antibody

CD154 is a member of the TNF family of molecules. It binds to CD40 and acts as a co-stimulatory molecule by activating antigen presenting cells, such as B cells. While CD154 is mostly found expressed on activated T cells.

Product Categories/Family for anti-CD40LG antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4557433
• NCBI GeneID: 959
• NCBI Accession #: NP_000065.1
• NCBI GenBank Nucleotide #: NM_000074.2
• UniProt Accession #: P29965
• Molecular Weight: 29,274 Da
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: CD40LG
• NCBI Official Synonym Symbols: IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• NCBI Protein Information: CD40 ligand; CD40-L; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
• UniProt Protein Name: CD40 ligand
• Protein Family: CD40 ligand
• UniProt Gene Name: CD40LG
• UniProt Synonym Gene Names: CD40L; TNFSF5; TRAP; CD40-L; TRAP
• UniProt Entry Name: CD40L_HUMAN

NCBI Description

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; leukocyte adhesion; regulation of immune response; positive regulation of interleukin-12 production; immunoglobulin secretion; B cell differentiation; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 1