Flow Cytometry (FC/FACS)
(Surface staining of ConA stimulated mouse splenocytes with CD153 antibody (PE). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.)
Rat anti-Mouse CD153 Monoclonal Antibody | anti-CD153 antibody
CD153 antibody
Gene Names
Tnfsf8; CD153; Cd30l; CD30LG; Tnlg3a
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
Purity
Purified by affinity chromatography.
Synonyms
CD153; Monoclonal Antibody; CD153 antibody; Rat monoclonal CD153 antibody; Monoclonal CD153 antibody; Anti-CD153 antibody; CD30 Ligand antibody; CD 153 antibody; CD-153; CD30L antibody; TNFSF8 antibody; CD-153 antibody; CD 153; anti-CD153 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
RM153
Specificity
Mouse
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
1
Applicable Applications for anti-CD153 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
FC/FACS: = 0.06ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Surface staining of ConA stimulated mouse splenocytes with CD153 antibody (PE). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Surface staining of ConA stimulated mouse splenocytes with CD153 antibody (PE). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.)
Related Product Information for anti-CD153 antibody
The RM153 monoclonal antibody reacts with mouse CD153. This 40 kDa cell surface receptor, a type II transmembrane protein, is a member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation. Expression of CD153 on T cells does not appear to require costimulation through CD28 and is upregulated via TCR engagement and peaks 24-48 hours after activation. Signaling through CD153 is important in costimulation of pre-activated T cells.
Product Categories/Family for anti-CD153 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,519 Da
NCBI Official Full Name
CD153 protein, partial
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 8
NCBI Official Symbol
Tnfsf8
NCBI Official Synonym Symbols
CD153; Cd30l; CD30LG; Tnlg3a
NCBI Protein Information
tumor necrosis factor ligand superfamily member 8
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 8
UniProt Gene Name
Tnfsf8
UniProt Synonym Gene Names
Cd30l; Cd30lg; CD30-L
Uniprot Description
Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Research Articles on CD153
Similar Products
Product Notes
The CD153 tnfsf8 (Catalog #AAA5309276) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD153 antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD153 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP). FC/FACS: = 0.06ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the CD153 tnfsf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD153, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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