anti-Mouse CD152/CTLA-4 Monoclonal Antibody
Hamster Anti-Mouse CD152/CTLA-4-PE
Reactivity
Mouse
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
CD152/CTLA-4; Monoclonal Antibody; Hamster Anti-Mouse CD152/CTLA-4-PE; Hamster Anti-Mouse CD152/CTLA-4 - Phycoerythrin-Labeled; Hamster Anti-Mouse CD152/CTLA-4; anti-CD152/CTLA-4 antibody
Host
Armenian Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Armenian Hamster IgG
Clone Number
1B8
Specificity
Mouse CD152/CTLA-4
The lymphocyte surface antigen CD152, also known as CTLA-4, is related to the co-stimulatory molecule CD28, and both molecules share common B7 family counter-receptors.1-4 However CD152 is thought to be a negative regulator of T cell activation1-3 and may play a role in apoptotic control of T cells. CD152 exists as a 69 kDa homodimer1, and is relatively conserved among humans, mice and chickens.2
The lymphocyte surface antigen CD152, also known as CTLA-4, is related to the co-stimulatory molecule CD28, and both molecules share common B7 family counter-receptors.1-4 However CD152 is thought to be a negative regulator of T cell activation1-3 and may play a role in apoptotic control of T cells. CD152 exists as a 69 kDa homodimer1, and is relatively conserved among humans, mice and chickens.2
Form/Format
R - phycoerythrin (R - PE) Conjugate
Applicable Applications for anti-CD152/CTLA-4 antibody
Flow Cytometry; Immunoprecipitation
Application Notes
Flow Cytometry Suggested Dilution: <= 0.3 ug/10^6 cells
Characterization
To insure acceptable performance, each batch of product is tested to conform to the characteristics of a standard reference reagent by flow cytometry.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Walunas, T. L., et al. 1994. Immunity 1:405.
2. Perkins, D., et al. 1996. J. Immunol. 156:4154.
3. Leach, D. R., M.K. Krummel and J.P. Allison. 1996. Science 271:1734.
4. Freeman, G. J., et al. 1992. J. Immunol. 149:3795.
2. Perkins, D., et al. 1996. J. Immunol. 156:4154.
3. Leach, D. R., M.K. Krummel and J.P. Allison. 1996. Science 271:1734.
4. Freeman, G. J., et al. 1992. J. Immunol. 149:3795.
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Product Notes
The CD152/CTLA-4 (Catalog #AAA673516) is an Antibody produced from Armenian Hamster and is intended for research purposes only. The product is available for immediate purchase. The Hamster Anti-Mouse CD152/CTLA-4-PE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD152/CTLA-4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation. Flow Cytometry Suggested Dilution: <= 0.3 ug/10^6 cells. Researchers should empirically determine the suitability of the CD152/CTLA-4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD152/CTLA-4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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