Mouse anti-Human CD15 Monoclonal Antibody | anti-CD15 antibody

Anti-Hu CD15 Purified

Cat. Num. AAA1801815

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified by protein-A affinity chromatography
• Synonyms: CD15; Monoclonal Antibody; Anti-Hu CD15 Purified; Lewis x Blood Group antigen; Le(x); SSEA-1; 3-fucosyl-N-acetyllactosamine; anti-CD15 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: W6D3
• Specificity: Mouse monoclonal antibody W6D3 recognizes an extracellular epitope of CD15, a cell membrane 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. This is a non-IgM anti-CD15 antibody.
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD15 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml
• Immunogen: WERI-RB-1 retinoblastoma cell line
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD15 (W6D3) purified antibody (concentration in sample 15 ug/ml, GAM APC).)

Flow Cytometry (FC/FACS)

(Separation of neutrophil granulocytes stained anti-human CD15 (W6D3) purified antibody (concentration in sample 15 ug/ml, GAM APC, red-filled) from neutrophil granulocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).)

Related Product Information for anti-CD15 antibody

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm identification of Hodgkin's disease.

References

*Mandalasi M, Dorabawila N, Smith DF, Heimburg-Molinaro J, Cummings RD, Nyame AK: Development and characterization of a specific IgG monoclonal antibody toward the Lewis x antigen using splenocytes of Schistosoma mansoni-infected mice. Glycobiology. 2013 Jul;23(7):877-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/23542315,*Sutherland DR, Ortiz F, Quest G, Illingworth A, Benko M, Nayyar R, Marinov I: High-sensitivity 5-, 6-, and 7-color PNH WBC assays for both Canto II and Navios platforms. Cytometry B Clin Cytom. 2018 Jul;94(4):637-651., URL: https://www.ncbi.nlm.nih.gov/pubmed/29381839
*Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep;39(1-2):185-94., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov;24(11):771-6., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1;406(2):333-41., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=