Mouse CD146 Monoclonal Antibody | anti-CD146 antibody

MOUSE ANTI HUMAN CD146:Preservative Free

Cat. Num. AAA210213

• Gene Names: MCAM; CD146; MUC18
• Applications: Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
• Synonyms: CD146; Monoclonal Antibody; MOUSE ANTI HUMAN CD146:Preservative Free; anti-CD146 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: OJ79c
• Form/Format: Preservative Free; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 646

• Applicable Applications for anti-CD146 antibody: Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Histology Positive Control Tissue: Melanoma; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Published customer image; Conjugated and unconjugated anti-CD326, anti-CD146 and anti-CD104 were analysed by flow cytometry for binding to CORL23 and LoVo cells [black line, negative control; blue line, unconjugated antibody; red line, immunoconjugates with porphyrin 1 (a) or porphyrin 2 (b)].From: Smith K, Malatesti N, Cauchon N, Hunting D, Lecomte R, van Lier JE, Greenman J, Boyle RW. Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology. 2011 Feb;132(2):256-65.)

Testing Data

(Published customer image: Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors. (A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-beta, RGS5, CD146, Nestin, a-SMA and NG2). They do not express hematopoietic (CD45), endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers (CD56), (green = isotype, red = respective marker).From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012) The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells. PLoS ONE 7(4): e35577.)

Related Product Information for anti-CD146 antibody

Mouse anti Human CD146 antibody, clone OJ79c recognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single pass type 1 transmembrane glycoprotein with a calculated molecular mass of ~72 kDa. However due to extensive N-linked glycosylation CD146 migrates in polyacrylamide gels with an apparent molecular mass of ~:118 kDa. CD146 is a member of the immunoglobulin superfamily bearing 2 V-type Ig-like and 3 C-type Ig-like domains. CD146 is expressed by all endothelial cells and by melanoma cells and appears to act as an adhesion molecule (UniProt: P43121). Expression in melanoma may be linked to tumor progression (Lehmann et al. 1989). Mouse anti Human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry (Chen et al. 2014).

NCBI and Uniprot Product Information

• NCBI GI #: 71274107
• NCBI GeneID: 4162
• NCBI Accession #: NP_006491.2
• NCBI GenBank Nucleotide #: NM_006500.2
• UniProt Accession #: P43121; O95812; Q59E86; Q6PHR3; Q6ZTR2
• Molecular Weight: 57,605 Da
• NCBI Official Full Name: cell surface glycoprotein MUC18
• NCBI Official Synonym Full Names: melanoma cell adhesion molecule
• NCBI Official Symbol: MCAM
• NCBI Official Synonym Symbols: CD146; MUC18
• NCBI Protein Information: cell surface glycoprotein MUC18; Gicerin; S-endo 1 endothelial-associated antigen; cell surface glycoprotein P1H12; melanoma adhesion molecule; melanoma-associated antigen A32; melanoma-associated antigen MUC18
• UniProt Protein Name: Cell surface glycoprotein MUC18
• UniProt Gene Name: MCAM
• UniProt Synonym Gene Names: MUC18
• UniProt Entry Name: MUC18_HUMAN

Uniprot Description

MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; focal adhesion; plasma membrane; integral to membrane; nucleus; external side of plasma membrane

Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration