Flow Cytometry (FC/FACS)
(Staining of bEND.3 cell line with 0.125 ug of Rat IgG1 K isotype Contol (PE) (blue histogram) or 0.125 ug of CD144 antibody (PE) (purple histogram). Total viable cells were used for analysis.)
Rat CD144 Monoclonal Antibody | anti-CD144 antibody
CD144 antibody (PE)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD144; Monoclonal Antibody; CD144 antibody (PE); Monoclonal CD144; Anti-CD144; CD 144; CD-144; Vascular endothelial cadherin; Cdh5; Cadherin 5; anti-CD144 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
BV13
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Applicable Applications for anti-CD144 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: 20 ul (0.06 ug)/100 ul blood (or per 1 million cells in 100 ul total staining volume)
Biological Significance
The BV13 monoclonal antibody reacts with mouse VE-Cadherin. VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains, and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros have the potential to give rise to hematopoietic cells.
Tag/Conjugate
PE
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of bEND.3 cell line with 0.125 ug of Rat IgG1 K isotype Contol (PE) (blue histogram) or 0.125 ug of CD144 antibody (PE) (purple histogram). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of bEND.3 cell line with 0.125 ug of Rat IgG1 K isotype Contol (PE) (blue histogram) or 0.125 ug of CD144 antibody (PE) (purple histogram). Total viable cells were used for analysis.)
Related Product Information for anti-CD144 antibody
Rat monoclonal CD144 antibody (PE)
Product Categories/Family for anti-CD144 antibody
Similar Products
Product Notes
The CD144 (Catalog #AAA834184) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD144 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: 20 ul (0.06 ug)/100 ul blood (or per 1 million cells in 100 ul total staining volume). Researchers should empirically determine the suitability of the CD144 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD144, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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