Typical Testing Data/Standard Curve (for reference only)
Guinea Pig Desmoglein 1 ELISA Kit | DSG1 elisa kit
Guinea pig Desmoglein 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: DSG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DSG1 antibody and an DSG1-HRP conjugate. The assay sample and buffer are incubated together with DSG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DSG1 concentration since DSG1 from samples and DSG1-HRP conjugate compete for the anti-DSG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DSG1 from the sample, fewer sites are left to bind DSG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DSG1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. The protein encoded by this gene has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus. [provided by RefSeq, Jul 2008]
Uniprot Description
DSG1: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1); also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 18q12.1
Cellular Component: desmosome; internal side of plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; cytosol; lateral plasma membrane
Molecular Function: toxin binding; gamma-catenin binding; calcium ion binding
Biological Process: intercellular junction assembly; cell-cell adhesion; maternal process involved in pregnancy; protein stabilization; apoptosis; calcium-dependent cell-cell adhesion; homophilic cell adhesion; response to progesterone stimulus; cell structure disassembly during apoptosis
Disease: Palmoplantar Keratoderma I, Striate, Focal, Or Diffuse; Erythroderma, Congenital, With Palmoplantar Keratoderma, Hypotrichosis, And Hyper-ige
Research Articles on DSG1
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Product Notes
The Guinea Pig DSG1 dsg1 (Catalog #AAA728685) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728685 ELISA Kit recognizes Guinea Pig DSG1. It is sometimes possible for the material contained within the vial of "Desmoglein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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