Testing Data
(Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone Tük4(MBS212217) as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively)
Mouse CD14 Monoclonal Antibody | anti-CD14 antibody
MOUSE ANTI HUMAN CD14
Purified IgG - liquid
Western Blotting: Application Note: MEM-18 recognizes CD14 under non-reducing conditions
Preparation
Shelf Life: 18 months from date of despatch.
Testing Data
(Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone Tük4(MBS212217) as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively)
Testing Data
(Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone Tük4(MBS212217) as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively)
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITC (MBS210867))
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITC (MBS210867))
Testing Data
(Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone UCHM1(MBS213102) as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively)
Testing Data
(Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone UCHM1(MBS213102) as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively)
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE (MBS213741))
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE (MBS213741))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
Uniprot Description
CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; endosome membrane; lipid raft
Molecular Function: peptidoglycan receptor activity; protein binding; opsonin receptor activity; lipopolysaccharide binding
Biological Process: I-kappaB kinase/NF-kappaB cascade; apoptosis; MyD88-independent toll-like receptor signaling pathway; positive regulation of endocytosis; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; phagocytosis; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to magnesium ion; response to ethanol; cell surface receptor linked signal transduction; response to heat; toll-like receptor signaling pathway; innate immune response; response to electrical stimulus; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of cytokine secretion
Research Articles on CD14
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Product Notes
The CD14 cd14 (Catalog #AAA212551) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)*. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Western Blotting: Application Note: MEM-18 recognizes CD14 under non-reducing conditions. Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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