Mouse anti-Human CD138 Monoclonal Antibody

CD138, CT (SDC1, SDC, Syndecan-1, CD_antigen=CD138, Flags: Precursor)

Cat. Num. AAA6009738

• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Ascites; Ascites
• Synonyms: CD138; Monoclonal Antibody; CT (SDC1; SDC; Syndecan-1; CD_antigen=CD138; Flags: Precursor); Anti -CD138; anti-CD138 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 587CT7.3.6.5
• Specificity: Human
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid, crude ascites with 0.09% sodium azide.

• Applicable Applications for anti-CD138 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence, Flow Cytometry, ELISA.; Dilution: ELISA: 1:1,000; Western Blot: 1:500~8000; Flow Cytometry 1:10~50; Immunofluorescenc 1:10~50
• Immunogen: CD138 Mab is generated from mouses immunized with a KLH conjugated synthetic peptide selected from human CD138.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(CD138 Antibody (C-term)(Ascites) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).)

Flow Cytometry (FC/FACS)

(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of U266 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)

Flow Cytometry (FC/FACS)

(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)

Flow Cytometry (FC/FACS)

(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with U266 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CD138 antibody

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.

Product Categories/Family for anti-CD138 antibody

Antibodies; Abs to CD Markers