Rat CD137 Monoclonal Antibody | anti-CD137 antibody

CD137, Mouse

Cat. Num. AAA4381535

• Gene Names: Tnfrsf9; ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
• Reactivity: Mouse; Does not react with Human.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD137; Monoclonal Antibody; Mouse; 4-1BB ligand receptor; 4-1BB; homolog; HLDA VI; induced by lymphocyte activation (ILA); Interleukin activated receptor homolog of Mouse Ly63; Ly63; Receptor protein 4 1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNF receptor superfamily member 9; Tumor necrosis factor receptor superfamily member 9 (TNFRSF9); anti-CD137 antibody

• Host: Rat
• Reactivity: Mouse; Does not react with Human.
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: LOB12
• Specificity: CD137 (4-1BB), a member of the tumor necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival. The LOB12.3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling and delay tumor growthin vivowhen administered in combination with immune checkpoint inhibitors.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 256

• Applicable Applications for anti-CD137 antibody: Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Functional Studies: Order Antibody without BSA & Azide; FC/FACS: 0.5-1ug/million cells in 0.1ml; IF: 0.5-1ug/ml
• Positive Control: MPBMC's or Thymus.
• Immunogen: Murine CD137 human Fc fusion protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 4 E2; 4 81.52 cM (Mouse)
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

1. Taraban, V. Y., et al. (2002). Expression and costimulatory effects of the TNF receptor superfamily members CD134 (OX40) and CD137 (4-1BB), and their role in the generation of anti-tumor immune responses. Eur J Immunol 32(12): 3617-3627. 2. Bartkowiak, T., et al. (2015). Unique potential of 4-1BB agonist antibody to promote durable regression of HPV+ tumors when combined with an E6/E7 peptide vaccine. Proc Natl Acad Sci U S A 112(38): E5290-5299.

NCBI and Uniprot Product Information

• NCBI GI #: 117422439
• NCBI GeneID: 21942
• NCBI Accession #: NP_001070977.1
• NCBI GenBank Nucleotide #: NP_001070977.1
• UniProt Accession #: P20334
• Molecular Weight: 39kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 9 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 9
• NCBI Official Symbol: Tnfrsf9
• NCBI Official Synonym Symbols: ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 9
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 9
• UniProt Gene Name: Tnfrsf9
• UniProt Synonym Gene Names: Cd137; Ila; Ly63
• UniProt Entry Name: TNR9_MOUSE

Uniprot Description

TNFRSF9: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: external side of plasma membrane; extracellular space; integral to plasma membrane

Molecular Function: cytokine binding; receptor activity; tumor necrosis factor receptor activity

Biological Process: immune response; induction of apoptosis via death domain receptors; inflammatory response; multicellular organismal development; regulation of apoptosis; regulation of cell proliferation; response to lipopolysaccharide