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Western Blot (WB) (Figure-1: Westernblot analysis of CD137. Anti- CD137 (Clone: ABM3D3.2E8) was tested at 4 ug/ml on Jurkat, HepG2 and Molt-4.)

Mouse anti-Human CD137 Monoclonal Antibody | anti-CD137 antibody

Monoclonal Antibody to CD137 (Clone: ABM3D3.2E8)

Gene Names
TNFRSF9; ILA; 4-1BB; CD137; CDw137
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Purified
Protein G Chromatography
Synonyms
CD137; Monoclonal Antibody; Monoclonal Antibody to CD137 (Clone: ABM3D3.2E8); Q07011; CD137 Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; T-cell antigen 4-1BB homolog; T-cell antigen ILA; anti-CD137 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, Kappa
Clone Number
ABM3D3.2E8
Purity/Purification
Purified
Protein G Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
255
Applicable Applications for anti-CD137 antibody
Immunohistochemistry (IHC), FACS, Western Blot (WB)
Application Notes
Western blot analysis: 4-6 ug/ml
Immunohistochemical Analysis: 2-4 ug/ml
Facs anaysis: 1-2 ug/10^6 Cells
Antigen
CD137
Immunogen Information
A partial length recombinant protein of CD137 was used as the immunogen for this antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4°C; stable for 6 months.
For long-term storage; store at -20°C.
Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Figure-1: Westernblot analysis of CD137. Anti- CD137 (Clone: ABM3D3.2E8) was tested at 4 ug/ml on Jurkat, HepG2 and Molt-4.)

Western Blot (WB) (Figure-1: Westernblot analysis of CD137. Anti- CD137 (Clone: ABM3D3.2E8) was tested at 4 ug/ml on Jurkat, HepG2 and Molt-4.)

Immunohistochemistry (IHC)

(Figure-2: Immunohistochemical analysis of CD137. Anti-CD137 (Clone: ABM3D3.2E8) was used in Human Brain tissue at 2 ug/ml.)

Immunohistochemistry (IHC) (Figure-2: Immunohistochemical analysis of CD137. Anti-CD137 (Clone: ABM3D3.2E8) was used in Human Brain tissue at 2 ug/ml.)

Immunohistochemistry (IHC)

(Figure-3: Immunohistochemical analysis of CD137. Anti-CD137 (Clone: ABM3D3.2E8) was used in Human Tonsil tissue at 2 ug/ml.)

Immunohistochemistry (IHC) (Figure-3: Immunohistochemical analysis of CD137. Anti-CD137 (Clone: ABM3D3.2E8) was used in Human Tonsil tissue at 2 ug/ml.)

Testing Data(TD)

(Figure-1: Cell surface staining of PHA stimulated PBMC. Green represents isotype control, red represents Anti-CD137 (MBS669040). 1 ug antibody was used. Goat anti mouse PE was used as secondary antibody.)

Testing Data(TD) (Figure-1: Cell surface staining of PHA stimulated PBMC. Green represents isotype control, red represents Anti-CD137 (MBS669040). 1 ug antibody was used. Goat anti mouse PE was used as secondary antibody.)
Product Categories/Family for anti-CD137 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 9
NCBI Official Synonym Full Names
TNF receptor superfamily member 9
NCBI Official Symbol
TNFRSF9
NCBI Official Synonym Symbols
ILA; 4-1BB; CD137; CDw137
NCBI Protein Information
tumor necrosis factor receptor superfamily member 9
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 9
UniProt Gene Name
TNFRSF9
UniProt Synonym Gene Names
CD137; ILA

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Research Articles on CD137

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Product Notes

The CD137 tnfrsf9 (Catalog #AAA669040) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to CD137 (Clone: ABM3D3.2E8) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD137 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FACS, Western Blot (WB). Western blot analysis: 4-6 ug/ml Immunohistochemical Analysis: 2-4 ug/ml Facs anaysis: 1-2 ug/10^6 Cells. Researchers should empirically determine the suitability of the CD137 tnfrsf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD137, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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