Rabbit anti-Human, Mouse USP28 Polyclonal Antibody | anti-USP28 antibody

USP28 Polyclonal Antibody

Cat. Num. AAA3009596

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: USP28; Polyclonal Antibody; USP28 Polyclonal Antibody; Ubiquitin carboxyl-terminal hydrolase 28; Deubiquitinating enzyme 28 Ubiquitin thioesterase 28; Ubiquitin-specific-processing protease 28; KIAA1515; anti-USP28 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: USP28 polyclonal antibody detects endogenous levels of USP28 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1077

• Applicable Applications for anti-USP28 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human USP28
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using USP28 antibody.)

Related Product Information for anti-USP28 antibody

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).

NCBI and Uniprot Product Information

• NCBI GI #: 16507200
• NCBI GeneID: 57646
• NCBI Accession #: NP_065937.1
• NCBI GenBank Nucleotide #: NP_065937.1
• UniProt Accession #: Q96RU2
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 28 isoform a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 28
• NCBI Official Symbol: USP28
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 28
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 28
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP28
• UniProt Synonym Gene Names: KIAA1515
• UniProt Entry Name: UBP28_HUMAN

NCBI Description

The protein encoded by this gene is a deubiquitinase involved in the DNA damage pathway and DNA damage-induced apoptosis. Overexpression of this gene is seen in several cancers. [provided by RefSeq, Oct 2016]

Uniprot Description

USP28: a peptidase of the C19 family. USP28 and FBXW7 regulate Myc protein stability in response to DNA damage. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; DNA damage checkpoint; response to ionizing radiation; DNA repair; response to DNA damage stimulus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis