Mouse anti-Human CD137 Monoclonal Antibody | anti-CD137 antibody

Mouse Anti Human CD137: APC

Cat. Num. AAA225747

• Gene Names: TNFRSF9; ILA; 4-1BB; CD137; CDw137
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: >95% by SDS PAGE; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Synonyms: CD137; Monoclonal Antibody; Mouse Anti Human CD137: APC; CD137 antibody; 4B4-1; anti-CD137 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4B4-1
• Purity/Purification: >95% by SDS PAGE; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Form/Format: Purified IgG conjugated to Allophycocyanin (APC) - Liquid
• Sequence Length: 255

• Applicable Applications for anti-CD137 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: Max Dilution: Neat; Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
• Immunogen: Recombinant human CD137 ectodomain
• Preservative Stabilizers: <0.1% Sodium Azide (NaN3) 0.2% Bovine Serum Albumin
• Buffer Solution: Phosphate Buffered Saline
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE.; This product should be stored undiluted.; This product is photosensitive and should be protected from light.

Testing Data

(Surface Staining of PHA activated PBMC with anti-CD137:RPE )

Related Product Information for anti-CD137 antibody

Mouse anti Human CD137, clone 4B4-1 recognizes CD137. CD137 (also known as 4-1BB ligand receptor or TNFRSF9) is a type I transmembrane glycoprotein of the Tumor Necrosis Factor receptor family that is expressed on activated T and NK cells, dendritic cells, myeloid cells and at the surface of some non-lymphoid cells under specific conditions (Schwarz et al. 1995). CD137 acts as a receptor for 4-1BB ligand, expressed on activated macrophages, activated B cells, hematopoietic stem cells and myeloid progenitor cells (Pollock et al. 1994) and for some proteins of the extracellular matrix (Chalupny et al. 1992).
The interaction between CD137 and 4-1BBL acts as a co-stimulatory signal for the activation, expansion and survival of T cells (Wen et al. 2002). CD137 signaling is involved in the generation of CD8+ T cells and enhance their cytotoxic activity (Shuford et al. 1997). Because of its ability to promote the anti-tumorigenic activity of cytotoxic T cells, CD137 is the target of several cancer immune therapies, such as the agonist anti-CD137 Urelumab (Segal et al. 2017) and Utomilumab (Chester et al. 2018).CD137 also binds to the adaptor proteins TRAF1, 2 and 3 to promote NF-kappaB activation (Jang et al. 1998).
Mouse anti-human CD137, clone 4B4-1 has been reported to immunoprecipitate a homodimeric protein of 32kDa under reducing conditions and 85kDa under non-reducing conditions (Garni-Wagner et al. 1996).

NCBI and Uniprot Product Information

• NCBI GI #: 5730095
• NCBI GeneID: 3604
• NCBI Accession #: NP_001552.2
• NCBI GenBank Nucleotide #: NP_001552.2
• UniProt Accession #: Q07011
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 9
• NCBI Official Synonym Full Names: TNF receptor superfamily member 9
• NCBI Official Symbol: TNFRSF9
• NCBI Official Synonym Symbols: ILA; 4-1BB; CD137; CDw137
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 9
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 9
• UniProt Gene Name: TNFRSF9
• UniProt Synonym Gene Names: CD137; ILA
• UniProt Entry Name: TNR9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF9: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function: cytokine binding; receptor activity

Biological Process: negative regulation of cell proliferation; apoptosis