Mouse CD137 Monoclonal Antibody | anti-CD137 antibody
anti-human CD137-Purified Preservative Free
Reactivity
Human, Monkey
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified Preservative Free
Synonyms
CD137; Monoclonal Antibody; anti-human CD137-Purified Preservative Free; Monoclonal anti-human CD137 (4-1BB); anti-CD137 antibody
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
4B4-1
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
41
Applicable Applications for anti-CD137 antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Performance
Cultured human CEM were stimulated 1 day in the presence of PMA (10ng/ml) and ionomycin (1muM). Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 mul of anti-CD137 antibody at 10 mug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 38% subpopulation of the cells stained positive with a mean shift of 1.14 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 mum filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Ectodomain of recombinant human 4-1BB fusion protein
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Related Product Information for anti-CD137 antibody
Information: Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival. Antibody 4B4-1 recognizes the CD137 molecule of about 35 kd from activated T cells.
References
M.R. Alderson, et al, (1994) Eur J Immunol 24: 2219-2227. H.J. Gruss, and S.K. Dower, (1995) Blood 85: 3378-3404. B.A. Garni-Wagner, et al, (1996) Cellular Immunol 169: 91-98. H. Schwarz, et al, (1996) Blood 87: 2839-2845.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
4,045 Da
NCBI Official Full Name
CD137, partial
UniProt Protein Name
CD137
UniProt Gene Name
TNFRSF9
UniProt Entry Name
A9YYF3_MACFA
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Product Notes
The CD137 tnfrsf9 (Catalog #AAA666589) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD137-Purified Preservative Free reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD137 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the CD137 tnfrsf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD137, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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