Rat anti-Mouse CD13 Monoclonal Antibody | anti-CD13 antibody
CD13, Mouse, mAb ER-BMDM1
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD13; Monoclonal Antibody; Mouse; mAb ER-BMDM1; anti-CD13 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-CD13 antibody
F, Flow Cytometry (FC/FACS), P
Application Notes
Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-CD13 antibody
Product Description: The monoclonal antibody ER-BMDM1 is directed against a 160-kDa cell membrane-associated antigen with aminopeptidase N activity. Aminopeptidase N (APN) is also known as Alanyl Aminopeptidase (ANPEP), CD13 antigen or membrane protein p161. The protein consists of a short cyplasmic tail, a transmembrane region, a Ser/Thr region, and a zinc metalloproteinase domain. Widely expressed in many cells, tissues, and species, aminopeptidase N cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. It has putative involvement in several biological processes including antigen processing and presentation, cell adhesion, tumor invasion and metastasis and neurotransmitter degradation. The antigen recognized by the mocnoclonal antibody ER-BMDM1 is present on various mouse macrophage and dendritic cell subpopulations as well as on microvillous epithelia. The ER-BMDM1 antigen is expressed at increasing levels upon maturation of macrophages. Therefore the monoclonal antibody ER-BMDM1 is a useful marker for the identification of aminopeptidase N positive macrophages and dendritic cells in mouse, the expression of the ER-BMDM1 antigen arises after the monocytic stage of differentation: bone marrow cells and peripheral blood monocytes are ER-BMDM1 negative, whereas mature macrophages express the ER-BMDM1 antigen. A subpopulation of dendrocytes and their in vitro equivalents, the dendritic cells, are also ER-BMDM1 positive.
References
1. Leenen, P et al; The monoclonal antibody ER-BMDM1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidase activity. Eur J Immunol 1992, 22:1567 2. Allaerts, W et al; A population of interstitial cells in the anterior pituitary with a hematopoietic origin and a rapid turnover: a relationship with folliculo-stellate cells? J Neuroimmunol 1997, 78: 184 3. Leenen, P et al; Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover. J Immunol 1998, 160: 2166
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Product Notes
The CD13 (Catalog #AAA584332) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD13, Mouse, mAb ER-BMDM1 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD13 can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), P. Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the CD13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD13, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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