Rabbit anti-Human FGD6 Polyclonal Antibody | anti-FGD6 antibody

FGD6, CT (FGD6, KIAA1362, ZFYVE24, FYVE, RhoGEF and PH domain-containing protein 6, Zinc finger FYVE domain-containing protein 24) (Azide free) (HRP)

Cat. Num. AAA6298949

• Gene Names: FGD6; ZFYVE24
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FGD6; Polyclonal Antibody; CT (FGD6; KIAA1362; ZFYVE24; FYVE; RhoGEF and PH domain-containing protein 6; Zinc finger FYVE domain-containing protein 24) (Azide free) (HRP); Zinc finger FYVE domain-containing protein 24; anti-FGD6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FGD6 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FGD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1331-1359 amino acids from the C-terminal region of human FGD6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FGD6 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FGD6 antibody detected the FGD6 protein (arrow).)

Immunohistochemistry (IHC)

(FGD6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FGD6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-FGD6 antibody

Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.

Product Categories/Family for anti-FGD6 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005). Jin, J., et al. Curr. Biol. 14(16):1436-1450(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 154240686
• NCBI GeneID: 55785
• NCBI Accession #: NP_060821.3
• NCBI GenBank Nucleotide #: NM_018351.3
• UniProt Accession #: Q6ZV73; Q6ZR53; Q7Z2Z7; Q96D44; Q9NUR8; Q9P2I5
• Molecular Weight: 160,816 Da
• NCBI Official Full Name: FYVE, RhoGEF and PH domain-containing protein 6
• NCBI Official Synonym Full Names: FYVE, RhoGEF and PH domain containing 6
• NCBI Official Symbol: FGD6
• NCBI Official Synonym Symbols: ZFYVE24
• NCBI Protein Information: FYVE, RhoGEF and PH domain-containing protein 6; zinc finger FYVE domain-containing protein 24
• UniProt Protein Name: FYVE, RhoGEF and PH domain-containing protein 6
• Protein Family: FYVE, RhoGEF and PH domain-containing protein
• UniProt Gene Name: FGD6
• UniProt Synonym Gene Names: KIAA1362; ZFYVE24
• UniProt Entry Name: FGD6_HUMAN

Uniprot Description

FGD6: May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. May play a role in regulating the actin cytoskeleton and cell shape. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: ruffle; Golgi apparatus; cytoskeleton; lamellipodium; cytoplasm

Molecular Function: small GTPase binding; Rho guanyl-nucleotide exchange factor activity; metal ion binding; guanyl-nucleotide exchange factor activity

Biological Process: regulation of cell shape; filopodium formation; cytoskeleton organization and biogenesis; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity