CD11c Monoclonal Antibody | anti-CD11c antibody
HAMSTER ANTI MOUSE CD11c
Gene Names
Itgax; Cr4; N418; Cd11c; AI449405
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
Purified
Synonyms
CD11c; Monoclonal Antibody; HAMSTER ANTI MOUSE CD11c; anti-CD11c antibody
Clonality
Monoclonal
Isotype
IgG
Clone Number
N418
Specificity
Hamster anti Mouse CD11c antibody, clone N418 recognizes the murine homolog of human CD11c, also known as Integrin Alpha X, a 150/90 kDa member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells. Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
Purity/Purification
Purified
Form/Format
Purified IgG - liquid; Phosphate buffered saline
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD11c antibody
Flow Cytometry (FC), Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
FC: 1/25 - 1/100
Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Mouse
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
Mouse spleen dendritic cells.
Fusion Partners
Spleen cells from immunised Armenian Hamster were fused with cells of the Sp2/0 myeloma cell line.
Preparation and Storage
Store at +4°C or at -20°C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Related Product Information for anti-CD11c antibody
Hamster anti Mouse CD11c antibody, clone N418 recognizes the murine homolog of human CD11c, a 150/90kDa member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells. Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (Wang et al. 2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
integrin alpha-X
NCBI Official Synonym Full Names
integrin alpha X
NCBI Official Symbol
Itgax
NCBI Official Synonym Symbols
Cr4; N418; Cd11c; AI449405
NCBI Protein Information
integrin alpha-X; CD11 antigen-like family member C; CD11C (p150) alpha polypeptide; complement receptor 4; integrin aX; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95
UniProt Protein Name
Integrin alpha-X
UniProt Gene Name
Itgax
UniProt Entry Name
ITAX_MOUSE
Uniprot Description
ITGAX: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Belongs to the integrin alpha chain family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral
Cellular Component: cell surface; membrane; integral to membrane; integrin complex; external side of plasma membrane
Molecular Function: metal ion binding
Biological Process: integrin-mediated signaling pathway; heterotypic cell-cell adhesion; activated T cell proliferation; cell adhesion; defense response to virus
Research Articles on CD11c
Similar Products
Product Notes
The CD11c itgax (Catalog #AAA212119) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD11c can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Immunofluorescence (IF). FC: 1/25 - 1/100 Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD11c itgax for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11c, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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