Mouse CD11b Monoclonal Antibody | anti-CD11b antibody

CD11b (Cell Surface Glycoprotein MAC1 Alpha Subunit, Complement Component Receptor 3 Alpha, CR3A, ITGAM, MAC1A, MO1A, Neutrophil Adherence Receptor) (MaxLight 550)

Cat. Num. AAA6254437

• Gene Names: ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• Reactivity: Human, Monkey
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by protein G affinity chromatography from tissue culture supernatant.
• Synonyms: CD11b; Monoclonal Antibody; CD11b (Cell Surface Glycoprotein MAC1 Alpha Subunit; Complement Component Receptor 3 Alpha; CR3A; ITGAM; MAC1A; MO1A; Neutrophil Adherence Receptor) (MaxLight 550); Cell Surface Glycoprotein MAC1 Alpha Subunit; Neutrophil Adherence Receptor; anti-CD11b antibody

• Host: Mouse
• Reactivity: Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3H965
• Specificity: Recognizes human CD11b. Species Crossreactivity: Cynomolgus monkey, baboon, Rhesus monkey. Does not react with feline. Reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release
• Purity/Purification: Purified by protein G affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight550.

• Applicable Applications for anti-CD11b antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Rheumatoid synovial cells and human monocytes.
• Conjugate: MaxLight550
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of human peripheral blood granulocytes with C2262-40X.)

Testing Data

(Staining of human peripheral blood granulocytes with C2262-41J.)

Testing Data

(Staining of human peripheral blood granulocytes with C2262-40T.)

Product Categories/Family for anti-CD11b antibody

Antibodies; CD Markers

References

1. Yoshino, M. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110. 2. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocyte. Leukocyte Typing IV. Oxford University Press 1058-1063. 3. Dransfield, I. et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function. J. Cell Biol. 116: 1527-1535. 4. Malhotra, V. et al. (1986) Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3). Eur. J. Immunol. 16: 1117-1123. 5. Glasow, A. et al. (2005) Retinoids and myelomonocytic growth factors co-operatively activate RAR{alpha} and induce human myeloid leukemia cell differentiation via MAP kinase pathways. Blood 105: 341-9. 6. Rezzonico, R. et al. (2001) Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood 97: 2932-2940. 7. Rezzonico, R. et al. (2000) Engagement of CD11b and CD11c beta2 integrin by antibodies or soluble CD23 induces IL-1beta production on primary human monocytes through mitogen-activated protein kinase-dependent pathways. Blood 95: 3868-3877. 8. Canalli, A.A. et al. (2001) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin. Haematologica. 96: 526-33. 9. Patel, S. et al. (2009) Reconstituted high-density lipoprotein increases plasma high-density lipoprotein anti-inflammatory properties and cholesterol efflux capacity in patients with type 2 diabetes. J Am Coll Cardiol. 53: 962-71. 10. Stirling, R.G. et al. (2001) Interleukin-5 induces CD34(+) eosinophil progenitor mobilization and eosinophil CCR3 expression in asthma. Am J Respir Crit Care Med. 164: 1403-9. 11. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow. J Pharmacol Exp Ther. 321: 656-62. 12. Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells. Clin Exp Immunol. 130: 256-62. 13. Ramacciotti, E. et al. (2011) Evaluation of soluble p-selectin as a marker for the diagnosis of deep venous thrombosis. Clin Appl Thromb Hemost. 17: 425-31.

NCBI and Uniprot Product Information

• NCBI GI #: 224831239
• NCBI GeneID: 3684
• NCBI Accession #: NP_001139280.1
• NCBI GenBank Nucleotide #: NM_001145808.1
• UniProt Accession #: P11215; Q4VAK0; Q4VAK1; Q4VAK2
• Molecular Weight: 127,179 Da
• NCBI Official Full Name: integrin alpha-M isoform 1
• NCBI Official Synonym Full Names: integrin, alpha M (complement component 3 receptor 3 subunit)
• NCBI Official Symbol: ITGAM
• NCBI Official Synonym Symbols: CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• NCBI Protein Information: integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subuni
• UniProt Protein Name: Integrin alpha-M
• UniProt Gene Name: ITGAM
• UniProt Synonym Gene Names: CD11B; CR3A
• UniProt Entry Name: ITAM_HUMAN

NCBI Description

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; plasma membrane; integrin complex; nucleus; external side of plasma membrane

Molecular Function: heparan sulfate proteoglycan binding; heparin binding; protein binding; opsonin binding; protein heterodimerization activity; metal ion binding; glycoprotein binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; activated T cell proliferation; leukocyte migration during inflammatory response; cellular extravasation; toll-like receptor signaling pathway; innate immune response; cell adhesion; blood coagulation; toll-like receptor 4 signaling pathway; leukocyte migration

Disease: Systemic Lupus Erythematosus, Susceptibility To, 6