Mouse anti-Human CD11a (LFA-1 alpha) Monoclonal Antibody
Mouse Anti-Human CD11a/LFA-1a-FITC
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
CD11a (LFA-1 alpha); Monoclonal Antibody; Mouse Anti-Human CD11a/LFA-1a-FITC; Mouse Anti-Human CD11a/LFA-1a - FITC; Mouse Anti-Human CD11a (LFA-1 alpha); anti-CD11a (LFA-1 alpha) antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
38
Specificity
Human CD11a (LFA-1 alpha)
CD11a, also known as leukocyte function-associated antigen-1 (LFA-1 alpha), represents the 180 kDa integrin alpha L subunit which combines with the CD18 beta 2 integrin subunit to form the alpha L beta 2 integrin heterodimer. It is expressed on lymphocytes, granulocytes, monocytes, and macrophages and is upregulated on activated T cells. CD11a/LFA-1 alpha mediates adhesion of lymphocytes to vascular endothelium and is involved in costimulation.1-5
CD11a, also known as leukocyte function-associated antigen-1 (LFA-1 alpha), represents the 180 kDa integrin alpha L subunit which combines with the CD18 beta 2 integrin subunit to form the alpha L beta 2 integrin heterodimer. It is expressed on lymphocytes, granulocytes, monocytes, and macrophages and is upregulated on activated T cells. CD11a/LFA-1 alpha mediates adhesion of lymphocytes to vascular endothelium and is involved in costimulation.1-5
Form/Format
Fluorescein (FITC) Conjugate
Applicable Applications for anti-CD11a (LFA-1 alpha) antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes
Research Application(s): Studies in cell adhesion and cytotoxicity
Immunoprecipitation
Blocking studies
Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
Immunoprecipitation
Blocking studies
Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
Characterization
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin/azide-free antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of PBS; aliquot and freeze upon receipt.
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin/azide-free antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of PBS; aliquot and freeze upon receipt.
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
References
1. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD11a Section, Academic Press, New York, p. 156.
2. Dransfield, I., and N. Hogg. 1989. EMBO J. 12:3759.
3. Springer, T.A. 1990. Nature 346:425.
4. Springer, T.A. 1994. Cell 76:301.
5. Lub, M., Y. van Kooyk, and C.G. Figdor. 1995. Immunol. Today 16:479.
2. Dransfield, I., and N. Hogg. 1989. EMBO J. 12:3759.
3. Springer, T.A. 1990. Nature 346:425.
4. Springer, T.A. 1994. Cell 76:301.
5. Lub, M., Y. van Kooyk, and C.G. Figdor. 1995. Immunol. Today 16:479.
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Product Notes
The CD11a (LFA-1 alpha) (Catalog #AAA674104) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human CD11a/LFA-1a-FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11a (LFA-1 alpha) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation. Research Application(s): Studies in cell adhesion and cytotoxicity Immunoprecipitation Blocking studies Flow Cytometry Suggested Dilution: 10 uL/10^6 cells. Researchers should empirically determine the suitability of the CD11a (LFA-1 alpha) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11a (LFA-1 alpha), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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