Mouse anti-Human CD11a Monoclonal Antibody | anti-CD11a antibody

CD11a (Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide, Integrin alpha-L, ITGAL, Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain, LFA 1 alpha (LFA1A), Ly15, Ly21, Lymphocyte function associated antigen 1, p180) (HRP)

Cat. Num. AAA6122613

• Gene Names: ITGAL; CD11A; LFA-1; LFA1A
• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: CD11a; Monoclonal Antibody; CD11a (Antigen CD11A (p180); lymphocyte function associated antigen 1; alpha polypeptide; Integrin alpha-L; ITGAL; Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain; LFA 1 alpha (LFA1A); Ly15; Ly21; Lymphocyte function associated antigen 1; p180) (HRP); Antigen CD11A (p180); p180; anti-CD11a antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: CRIS-3
• Specificity: Recognizes human CD11a.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-CD11a antibody: Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP)
• Application Notes: IP: 0.5-1ug/500ug protein lysate; IHC-F: 0.5-1.0ug/ml for 30 minutes at RT; Applications are based on unconjugated antibody.
• Immunogen: Stimulated human leukocytes. Cellular Localization: Cell surface
• Conjugate: HRP
• Positive Control: JY, HUT-78, MOLT-4, HL60, U937, and human leukocytes. Human lymph nodes and tonsils.
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(FCM staining of human PBMCs using CD11a MAb (CRIS-3).)

Product Categories/Family for anti-CD11a antibody

Antibodies; CD Markers

References

1. Reinherz EL et al. eds. Leukocyte Typing II, Oxford University Press, Oxford, 1985 2. Knapp W. et al. Leukocyte Typing IV, Oxford Unioversity Press, Oxford, 1989. 3. Petruzzelli L et al. Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18. J Immunol 1995, 155(2):854-866. 4. Edwards CP et al. Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1.J Biol Chem 1998, 273(44):28937-44. 5. Shang XZ and Issekutz AC. Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol 1998, 28(6):1970-1979 6. Tian L et al. The neuronal glycoprotein telencephalin is a cellular ligand for the CD11a/CD18 leukocyte integrin. J Immunol 1997, 158(2):928-936.

NCBI and Uniprot Product Information

• NCBI GI #: 88911345
• NCBI GeneID: 3683
• UniProt Accession #: P20701; O43746; Q45H73; Q96HB1; Q9UBC8
• NCBI Official Full Name: Integrin alpha-L
• NCBI Official Synonym Full Names: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• NCBI Official Symbol: ITGAL
• NCBI Official Synonym Symbols: CD11A; LFA-1; LFA1A
• NCBI Protein Information: integrin alpha-L; LFA-1A; LFA-1 alpha; integrin gene promoter; CD11 antigen-like family member A; lymphocyte function-associated antigen 1; leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; antigen CD
• UniProt Protein Name: Integrin alpha-L
• UniProt Gene Name: ITGAL
• UniProt Synonym Gene Names: CD11A; LFA-1A
• UniProt Entry Name: ITAL_HUMAN

NCBI Description

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGAL: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Belongs to the integrin alpha chain family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane; immunological synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; ICAM-3 receptor activity; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; regulation of immune response; cell-matrix adhesion; activated T cell proliferation; positive regulation of calcium-mediated signaling; signal transduction; receptor clustering; heterophilic cell adhesion; leukocyte adhesion; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; positive regulation of T cell proliferation; inflammatory response; blood coagulation; cell adhesion; cell motility; leukocyte migration