Rabbit anti-Human OSBP Polyclonal Antibody | anti-OSBP antibody

OSBP Antibody

Cat. Num. AAA7113202

• Gene Names: OSBP; OSBP1
• Reactivity: Human
• Applications: ELISA, Immunofluorescence
• Purity: >95%; Protein G Purified
• Synonyms: OSBP; Polyclonal Antibody; OSBP Antibody; Oxysterol-binding protein 1; OSBP1; anti-OSBP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%; Protein G Purified
• Form/Format: Liquid
• Sequence Length: 807

• Applicable Applications for anti-OSBP antibody: ELISA (EIA), Immunofluorescence (IF)
• Application Notes: IF: 1:50-1:200
• Immunogen Species: Human
• Immunogen: Recombinant Human Oxysterol-binding protein 1 protein (628-802AA)
• Conjugate: Non-Conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Immunofluorescence (IF)

(Immunofluorescence staining of Hela cells with CSB-PA017254LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Product Categories/Family for anti-OSBP antibody

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4505531
• NCBI GeneID: 5007
• NCBI Accession #: NP_002547.1
• NCBI GenBank Nucleotide #: NM_002556.3
• UniProt Accession #: P22059; Q6P524
• Molecular Weight: 89,421 Da
• NCBI Official Full Name: oxysterol-binding protein 1
• NCBI Official Synonym Full Names: oxysterol binding protein
• NCBI Official Symbol: OSBP
• NCBI Official Synonym Symbols: OSBP1
• NCBI Protein Information: oxysterol-binding protein 1
• UniProt Protein Name: Oxysterol-binding protein 1
• Protein Family: Oxysterol-binding protein
• UniProt Gene Name: OSBP

NCBI Description

Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase [provided by RefSeq, Jul 2008]

Uniprot Description

Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum (PubMed:24209621). Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed:15746430, PubMed:17428193). Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed:15746430). Regulates cholesterol efflux by decreasing ABCA1 stability (PubMed:18450749).