Mouse anti-Human. CD104 Monoclonal Antibody | anti-ITGB4 antibody

CD104 (Integrin Beta-4)

Cat. Num. AAA438251

• Gene Names: ITGB4; CD104
• Reactivity: Human.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: CD104; Monoclonal Antibody; CD104 (Integrin Beta-4); GP150; Integrin beta-4; ITGB4; anti-ITGB4 antibody

• Host: Mouse
• Reactivity: Human.
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: UM-A9
• Specificity: Recognizes a protein of 205kDa, which is identified as integrin beta-4 (ITGB4). Its epitope is localized in the extracellular domain of ITGB4 protein. Integrins are heterodimers comprised of alpha and beta subunits, that are non-covalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta-4 subunit, a receptor for the laminins. This subunit tends to associate with alpha-6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1822

• Applicable Applications for anti-ITGB4 antibody: Biological Blockade, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)
• Application Notes: Biological Blockade (Partially blocks laminin binding) (Order Ab without Azide); ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Western Blotting (0.5-1ug/ml); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.
• Localization: Cell surface
• Positive Control: A431 cells, squamous cell carcinoma, colon or placenta
• Human Chromosomal Location: 17q25.1
• Immunogen: Human squamous cell carcinoma (UM-SCC1)
• Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months.

References

1. Signal transduction by the alpha 6 beta 4 integrin: distinct beta 4 subunit sites mediate recruitment of Shc/Grb2 and association with the cytoskeleton of hemidesmosomes. Mainiero F, Pepe A, Wary KK, Spinardi L, Mohammadi M, Schlessinger J, Giancotti FG. EMBO J. 1995;14(18):4470-81.
2. The A9 antigen associated with aggressive human squamous carcinoma is structurally and functionally similar to the newly defined integrin alpha 6 beta 4. Van Waes C, Kozarsky KF, Warren AB, Kidd L, Paugh D, Liebert M, Carey TE. Cancer Res. 1991;51(9):2395-402.
3. Altered expression in squamous carcinoma cells of an orientation restricted epithelial antigen detected by monoclonal antibody A9. Kimmel KA, Carey TE. Cancer Res. 1986 Jul;46(7):3614-23.

NCBI and Uniprot Product Information

• NCBI GI #: 54607035
• NCBI GeneID: 3691
• NCBI Accession #: NP_000204.3
• NCBI GenBank Nucleotide #: NM_000213.3
• UniProt Accession #: P16144; O14690; O14691; O15339; O15340; O15341; Q0VF97; Q9UIQ4; A0AVL6
• Molecular Weight: 205kDa
• NCBI Official Full Name: integrin beta-4 isoform 1
• NCBI Official Synonym Full Names: integrin, beta 4
• NCBI Official Symbol: ITGB4
• NCBI Official Synonym Symbols: CD104
• NCBI Protein Information: integrin beta-4; GP150; CD104 antigen; integrin beta-4 subunit
• UniProt Protein Name: Integrin beta-4
• Protein Family: Integrin
• UniProt Gene Name: ITGB4
• UniProt Entry Name: ITB4_HUMAN

NCBI Description

Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB4: integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Five alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cell surface; hemidesmosome; leading edge; plasma membrane; basal plasma membrane; basement membrane; integrin complex; receptor complex

Molecular Function: protein binding; G-protein-coupled receptor binding; receptor activity

Biological Process: integrin-mediated signaling pathway; skin development; extracellular matrix organization and biogenesis; hemidesmosome assembly; filopodium formation; gut development; cell-matrix adhesion; response to wounding; cell motility involved in cell locomotion; autophagy; mesodermal cell differentiation; cell adhesion

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Junctionalis With Pyloric Atresia