Rabbit anti-Human, Mouse MRPL12 Polyclonal Antibody | anti-MRPL12 antibody

MRPL12 Antibody

Cat. Num. AAA9605113

• Gene Names: MRPL12; 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: MRPL12; Polyclonal Antibody; MRPL12 Antibody; 39S ribosomal protein L12; mitochondrial; 5c5-2; FLJ60124; L12mt; MGC8610; Mitochondrial ribosomal protein L12; MRP-L12; MRP-L31/34; MRPL7; MRPL7/L12; RM12_HUMAN; RPML12; anti-MRPL12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MRPL12 antibody detects endogenous levels of total MRPL12
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 198

• Applicable Applications for anti-MRPL12 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Mitochondrion.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Xenopus
• Similarity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Xenopus (90%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from COLO cells, using MRPL12 antibody.)

Immunofluorescene (IF)

(MBS9605113 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-MRPL12 antibody

Function: Associates with mitochondrial RNA polymerase to activate transcription.
Subunit Structure: Component of the mitochondrial large ribosomal subunit (mt-LSU) (PubMed:8626705). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins (Probable). bL12m interacts with NOA1 (PubMed:19103604).
Similarity: Belongs to the bacterial ribosomal protein bL12 family.

NCBI and Uniprot Product Information

• NCBI GI #: 27436901
• NCBI GeneID: 6182
• NCBI Accession #: NP_002940.2
• NCBI GenBank Nucleotide #: NM_002949.3
• UniProt Accession #: P52815; Q969U0; Q9HCA2; Q9UQJ3
• Molecular Weight: Observed: 21 kD; Predicted: 22 kDa
• NCBI Official Full Name: 39S ribosomal protein L12, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L12
• NCBI Official Symbol: MRPL12
• NCBI Official Synonym Symbols: 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34
• NCBI Protein Information: 39S ribosomal protein L12, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L12, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL12
• UniProt Synonym Gene Names: MRPL7; RPML12; L12mt; MRP-L12

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq, Jul 2008]

Uniprot Description

Associates with mitochondrial RNA polymerase to activate transcription.