Rat anti-Mouse CD101 Monoclonal Antibody | anti-Cd101 antibody
CD101 (EWI-101, Glu-Trp-Ile EWI motif-containing protein 101, Gm1016, Gm734, Igsf2, IgSF2, Immunoglobulin superfamily member 2, LOC381460)
Gene Names
Cd101; Gm734; Igsf2; Gm1016; Cd101
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
CD101; Monoclonal Antibody; CD101 (EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; Gm1016; Gm734; Igsf2; IgSF2; Immunoglobulin superfamily member 2; LOC381460); Anti -CD101 (EWI-101; anti-Cd101 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7H22
Specificity
Selected for its ability to detect IGSF2 on mouse splenocytes in flow cytometry.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
Applicable Applications for anti-Cd101 antibody
Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry: Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 L of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome., Optimal dilutions should be determined by each laboratory for each application.
Immunogen
rmIGSF2 extracellular domian
Hybridoma
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified recombinant mouse IGSF2 (rmIGSF2; Accession # BAD97674) extracellular domain.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Cd101 antibody
IGSF2, also known as CD101, is an Ig-superfamily transmembrane protein. It is expressed on dendritic cells, Langerhans cells, granulocytes, and activated T cells. IGSF2 ligation is involved in T cell activation. Polymorphisms in IGSF2 are associated with susceptibility to type I diabetes. The extracellular domains of mouse and human IGSF2 share 70% amino acid sequence identity.
Product Categories/Family for anti-Cd101 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
114,205 Da
NCBI Official Full Name
CD101 protein
NCBI Official Synonym Full Names
CD101 antigen
NCBI Official Symbol
Cd101
NCBI Official Synonym Symbols
Gm734; Igsf2; Gm1016; Cd101
NCBI Protein Information
immunoglobulin superfamily member 2; EWI-101; leukocyte surface protein; immunoglobulin superfamily, member 2; EWI immunoglobulin subfamily member EWI-101; glu-Trp-Ile EWI motif-containing protein 101
UniProt Protein Name
Immunoglobulin superfamily member 2
Protein Family
UniProt Gene Name
Cd101
UniProt Synonym Gene Names
Igsf2
UniProt Entry Name
IGSF2_MOUSE
Uniprot Description
IGSF2: Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3- induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Cellular Component: membrane; integral to membrane
Biological Process: positive regulation of myeloid leukocyte differentiation
Research Articles on Cd101
1. of the eight genes found in the Idd10 region, the CD101 gene was selected as a candidate for Idd10 based on its function in humans and its polymorphism in the NOD strain of mice
2. Cd101, but not Fcgr1, is responsible for the Idd10 effect
2. Cd101, but not Fcgr1, is responsible for the Idd10 effect
Similar Products
Product Notes
The Cd101 cd101 (Catalog #AAA601720) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD101 (EWI-101, Glu-Trp-Ile EWI motif-containing protein 101, Gm1016, Gm734, Igsf2, IgSF2, Immunoglobulin superfamily member 2, LOC381460) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD101 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Flow Cytometry: Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 L of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the Cd101 cd101 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD101, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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