Mouse anti-Human CCL27 Monoclonal Antibody | anti-CCL27 antibody

Mouse anti Human CCL27

Cat. Num. AAA226095

• Gene Names: CCL27; ALP; ILC; CTAK; CTACK; PESKY; ESKINE; SCYA27
• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: CCL27; Monoclonal Antibody; Mouse anti Human CCL27; CCL27 antibody | A01-2H4; anti-CCL27 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: A01-2H4
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)

• Applicable Applications for anti-CCL27 antibody: ELISA (EIA)
• Application Notes: ELISA: 1.0ug/ml
• Immunogen: E Coli derived recombinant human full length CCL27 (Phe25-Gly112)
• Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human CCL27 using Mouse anti Human CCL27 (MBS226095) as a capture reagent and biotinylated Mouse anti Human CCL27 as a detection reagent with recombinant human CCL27 as the antigen. Detection was performed using HRP c)

Related Product Information for anti-CCL27 antibody

Mouse anti Human CCL27 antibody, clone A01-2H4, recognizes C-C motif chemokine 27, also known as CTACK, ESkine, ILC, and SCYA27. CCL27 is a C-C motif chemokine. CCR10 is the only known receptor for CCL27, and is expressed in normal skin (Mao et al. 2018). CCL27 is able to attract a subset of memory / effector T cells from peripheral blood to specific locations on the skin (Wang et al. 2018). CCL27 plays a role in T cell mediated skin inflammation, and high levels have been found in patients with psoraisis and atopic dermatitis. Upregulation of CCL27 has been identified in patients with myeloma, and is correlated with poor overall survival (Thangavadivel et al. 2016). CCL27 is secreted by primary tumor cells with high metastatic potential and is able to attract lymphatic endothelial cells, a crucial process during lymphangiogenesis (Karnezis et al. 2019).

This purified Mouse anti Human CCL27 antibody, clone A01-2H4 can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human CCL27 antibody, clone B05-10H9 as the detection antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 5730035
• NCBI GeneID: 10850
• NCBI Accession #: NP_006655.1
• NCBI GenBank Nucleotide #: NM_006664.2
• UniProt Accession #: Q9Y4X3
• Molecular Weight: 12,618 Da
• NCBI Official Full Name: C-C motif chemokine 27
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 27
• NCBI Official Symbol: CCL27
• NCBI Official Synonym Symbols: ALP; ILC; CTAK; CTACK; PESKY; ESKINE; SCYA27
• NCBI Protein Information: C-C motif chemokine 27; skinkine; CC chemokine ILC; IL-11 Ralpha-locus chemokine; small-inducible cytokine A27; IL-11 R-alpha-locus chemokine; cutaneous T-cell attracting chemokine; cutaneous T-cell-attracting chemokine; small inducible cytokine subfamily
• UniProt Protein Name: C-C motif chemokine 27
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL27
• UniProt Synonym Gene Names: ILC; SCYA27; CTACK
• UniProt Entry Name: CCL27_HUMAN

NCBI Description

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL27: Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. May play a role in cell migration during embryogenesis. Nuclear forms may facilitate cellular migration by inducing cytoskeletal relaxation. Binds to CCR10. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity

Biological Process: cell-cell signaling; immune response; chemotaxis