Mouse CBL Monoclonal Antibody | anti-CBL antibody

CBL (AP)

Cat. Num. AAA6241543

• Gene Names: CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Immunoprecipitation, Immunoblot, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: CBL; Monoclonal Antibody; CBL (AP); E3 ubiquitin-protein ligase CBL; Casitas B-lineage lymphoma proto-oncogene; CBL2; RNF55; C-CBL; anti-CBL antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 0.T.12
• Specificity: Specific for Cbl. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CBL antibody: Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunoblot
• Application Notes: ICC: 5-10ug/ml of a previous lot showed positive immunostaining for Cbl in 3T3 cells fixed with ice cold methanol/ethanol [1:1] for 1 minute.; IP: 2ug of a previous lot immunoprecipitated Cbl from 1ug of a mouse 3T3 cell RIPA lysate.; Immunoblot Analysis: 0.5-2ug/ml detects Cbl (120kD) in RIPA lysates from human Jurkat and mouse 3T3 cells. 3T3 cell lysate was resolved by electro-phoresis, transferred to nitrocellulose and probed with anti-Cbl (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.; Applications are based on unconjugated antibody.
• Immunogen: GST fusion protein corresponding to residues 878-906 of human Cbl. This sequence is identical in mouse.
• Conjugate: AP
• Preparation and Storage: Store at 4 degree C for short-term. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-CBL antibody

Antibodies; Oncoproteins

References

1. Blake, T.J. et al. (1991) Oncogene 6, 653-657. 2. Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482. 3. Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294-307. 4. Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323-8331. 5. Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339. 6. Hunter, S. et al. (1999) J. Biol. Chem. 274, 2097-2106.

NCBI and Uniprot Product Information

• NCBI GI #: 52426745
• NCBI GeneID: 867
• NCBI Accession #: NP_005179.2
• NCBI GenBank Nucleotide #: NM_005188
• UniProt Accession #: P22681; A3KMP8
• Molecular Weight: 99,633 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL
• NCBI Official Synonym Full Names: Cbl proto-oncogene, E3 ubiquitin protein ligase
• NCBI Official Symbol: CBL
• NCBI Official Synonym Symbols: CBL2; NSLL; C-CBL; RNF55; FRA11B
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL; oncogene CBL2; proto-oncogene c-Cbl; RING finger protein 55; signal transduction protein CBL; casitas B-lineage lymphoma proto-oncogene; fragile site, folic acid type, rare, fra(11)(q23.3); Cas-Br-M (murine) ecotropic retr
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL
• Protein Family: CBL-interacting protein kinase
• UniProt Gene Name: CBL
• UniProt Synonym Gene Names: CBL2; RNF55
• UniProt Entry Name: CBL_HUMAN

NCBI Description

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012]

Uniprot Description

CBL: an adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. Participates in signal transduction in hematopoietic cells.

Protein type: Ligase; EC 6.3.2.19; EC 6.3.2.-; Adaptor/scaffold; Oncoprotein; Motility/polarity/chemotaxis; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; plasma membrane; flotillin complex; nucleus; cytosol

Molecular Function: protein binding; signal transducer activity; ephrin receptor binding; zinc ion binding; phosphotyrosine binding; ubiquitin-protein ligase activity; calcium ion binding; SH3 domain binding; transcription factor activity; ligase activity

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; cell surface receptor linked signal transduction; fibroblast growth factor receptor signaling pathway; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; positive regulation of receptor-mediated endocytosis; negative regulation of apoptosis

Disease: Noonan Syndrome-like Disorder With Or Without Juvenile Myelomonocytic Leukemia