Mouse Cathepsin D Monoclonal Antibody | anti-CTSD antibody

Mouse Anti Human Cathepsin D

Cat. Num. AAA140196

• Gene Names: CTSD; CPSD; CLN10; HEL-S-130P
• Applications: ELISA, Western Blot
• Purity: CTSD antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Cathepsin D; Monoclonal Antibody; Mouse Anti Human Cathepsin D; CTSD Antibody; Mouse Anti Human; EC 3.4.23.5; CTSD; CPSD; CLN10; MGC2311; anti-CTSD antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b heavy chain and kappa light chain
• Clone Number: P4G2AT
• Purity/Purification: CTSD antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.; Sterile Filtered colorless solution.
• Sequence Length: 412

• Applicable Applications for anti-CTSD antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: CTSD antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
• Immunogen: Anti-human CTSD mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CTSD amino acids 21-412 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-CTSD antibody

Introduction: Cathepsin D is synthesized as a 54kDa precursor, which is proteolytically processed to an intermediate 48kDa single chain, which matures into more stable 34kDa and 14kDa two chain form. It is an estrogen-regulated lysosomal protease that has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and xonnective tissue. Because of its mitogenic and proteolytic activities, it has been implicated as a prognostic marker in many tumor types. Cathepsin D is expressed in epithelial cells as well as in macrophages.

Product Categories/Family for anti-CTSD antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 4503143
• NCBI GeneID: 1509
• NCBI Accession #: NP_001900.1
• NCBI GenBank Nucleotide #: NM_001909.4
• Molecular Weight: 44,552 Da
• NCBI Official Full Name: cathepsin D preproprotein
• NCBI Official Synonym Full Names: cathepsin D
• NCBI Official Symbol: CTSD
• NCBI Official Synonym Symbols: CPSD; CLN10; HEL-S-130P
• NCBI Protein Information: cathepsin D; ceroid-lipofuscinosis, neuronal 10; epididymis secretory sperm binding protein Li 130P; lysosomal aspartyl peptidase; lysosomal aspartyl protease
• UniProt Protein Name: Cathepsin D
• Protein Family: Cathepsin
• UniProt Gene Name: CTSD
• UniProt Synonym Gene Names: CPSD
• UniProt Entry Name: CATD_HUMAN

NCBI Description

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family.

Protein type: Autophagy; Protease; EC 3.4.23.5; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; lysosome; melanosome; extracellular region

Molecular Function: protein binding; aspartic-type endopeptidase activity

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis

Disease: Ceroid Lipofuscinosis, Neuronal, 10