Mouse Catenin, p120 Monoclonal Antibody | anti-CTNND1 antibody

Catenin, p120, CT

Cat. Num. AAA603147

• Gene Names: CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Ascites; Ascites
• Synonyms: Catenin; p120; Monoclonal Antibody; CT; Anti -Catenin; anti-CTNND1 antibody

• Host: Mouse
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3G58
• Specificity: Recognizes p120 Catenin proteins and can be used to detect isoforms 1-4 of the protein. Reacts with a C-terminal epitope found in all known p120 isoforms.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid, 0.05% sodium azide.

• Applicable Applications for anti-CTNND1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence (canine), ELISA (immunogen), Western Blot and Immunoprecipitation.; Dilution: Immunofluorescence: 1:250-1:500; ELISA: 1:1000-1:10,000; Western Blot: 1:1000; Immunoprecipitation (Native): 5-10ul/IP reaction
• Immunogen: Recombinant fusion protein derived from the C-terminus of murine p120 Catenin
• Positive Control: MDCK cells (canine), NIH 3T3 cells (mouse), Va-2 broblasts (human), HeLa cells (human), and rat-2 broblasts.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in buffer.

Related Product Information for anti-CTNND1 antibody

Originally characterized as a prominent substrate for Src (14) and receptor protein tyrosine kinases (eg, EGF, PDGF, CSF-1) (6), p120ctn is a novel catenin (10, 13) which binds directly to the juxta- membrane region of classical cadherins (eg, E-cadherin)(3,11,13,18). Emerging evidence suggests a prominent role in the regulation of cadherin mediated adhesion (1,4,12). Most cells express multiple isoforms of p120 that arise by alternative splicing (8, 9, 11), some of which can be distinguished using monoclonal antibodies (17). Fibroblasts express mostly type 1 isoforms containing an N-terminal extension of 101 amino acids. Type I isoforms may be important for cell motility and are selectively recognized. Epithelial cells preferentially express shorter isoforms lacking this extension. Heterogeneous or negative p120 staining has been reported in breast (5) and colon carcinomas (7, 16) and decreased p120 expression in bladder tumors correlated with a poor prognosis (15). Loss of p120 or E-cadherin never occurs in epithelial tissues under normal circumstances, and its expression is probably mandatory for normal tissue architecture. Like its cousin b-catenin, p120 may have dual roles in both cell-cell adhesion and in nuclear signaling.

Product Categories/Family for anti-CTNND1 antibody

Antibodies; Abs to Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 10835010
• NCBI GeneID: 1500
• NCBI Accession #: NP_001322.1
• NCBI GenBank Nucleotide #: NM_001331.2
• UniProt Accession #: O60716; O15088; O60713; O60714; O60715; O60935; Q6DHZ7; Q6RBX8; Q9UP71; Q9UP72; Q9UP73; A8K939
• Molecular Weight: 108,170 Da
• NCBI Official Full Name: catenin delta-1 isoform 1B
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), delta 1
• NCBI Official Symbol: CTNND1
• NCBI Official Synonym Symbols: CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
• NCBI Protein Information: catenin delta-1; p120 catenin; cadherin-associated Src substrate
• UniProt Protein Name: Catenin delta-1
• Protein Family: Catenin
• UniProt Gene Name: CTNND1
• UniProt Synonym Gene Names: KIAA0384; CAS; p120(ctn)
• UniProt Entry Name: CTND1_HUMAN

NCBI Description

This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CTNND1: an Arm repeat protein that interacts with varied components such as cadherin, small G proteins, kinases, and the Kaiso transcriptional repressor. A downstream modulator of Wnt/beta-catenin signaling pathway whose stability may be regulated downstream of Dsh. Required for the invasiveness of E-cadherin-deficient cells. A signal coordinator between cadherins and Rho-family GTPases to regulate cytoskeletal changes required during dendritic spine and synapse development. The association of catenins with cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Thirty two splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold; Actin-binding; G protein regulator, misc.; Cell adhesion

Chromosomal Location of Human Ortholog: 11q11

Cellular Component: growth cone; lamellipodium; cytoplasm; dendritic spine; plasma membrane; synapse; intercellular junction; zonula adherens; midbody; cytosol; nucleus

Molecular Function: protein binding; cadherin binding; protein kinase binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; brain development; vascular endothelial growth factor receptor signaling pathway; cell adhesion