Western Blot (WB)
(Western BlotPositive WB detected in: HL-60 whole cell lysate, LO2 whole cell lysateAll lanes: CASP9 antibody at 1.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47, 31, 18, 37 KDaObserved band size: 47 KDa)
CASP9 recombinant antibody
CASP9 Antibody
IF: 1:20-1:200
Western Blot (WB)
(Western BlotPositive WB detected in: HL-60 whole cell lysate, LO2 whole cell lysateAll lanes: CASP9 antibody at 1.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47, 31, 18, 37 KDaObserved band size: 47 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: HL-60 whole cell lysate, LO2 whole cell lysateAll lanes: CASP9 antibody at 1.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47, 31, 18, 37 KDaObserved band size: 47 KDa)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with CSB-RA004555A0HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with CSB-RA004555A0HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Flow Cytometry (FC/FACS)
(Overlay histogram showing K562 cells stained with CSB-RA004555A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing K562 cells stained with CSB-RA004555A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Uniprot Description
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Research Articles on CASP9
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Product Notes
The CASP9 casp9 (Catalog #AAA7114792) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CASP9 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CASP9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:500-1:5000 IF: 1:20-1:200. Researchers should empirically determine the suitability of the CASP9 casp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASP9, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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