Rat anti-Human, Mouse CASP10 Monoclonal Antibody | anti-CASP10 antibody

CASP10, CT (Caspase-10, CASP-10, ICE-like Apoptotic Protease 4, Apoptotic Protease Mch-4, FAS-associated Death Domain Protein Interleukin-1B-converting Enzyme 2, FLICE2, Caspase-10 Subunit p23/17, Caspase-10 Subunit p12, MCH4)

Cat. Num. AAA648344

• Gene Names: CASP10; MCH4; ALPS2; FLICE2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CASP10; Monoclonal Antibody; CT (Caspase-10; CASP-10; ICE-like Apoptotic Protease 4; Apoptotic Protease Mch-4; FAS-associated Death Domain Protein Interleukin-1B-converting Enzyme 2; FLICE2; Caspase-10 Subunit p23/17; Caspase-10 Subunit p12; MCH4); Anti -CASP10; anti-CASP10 antibody

• Host: Rat
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: Recognizes human Caspase-10. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CASP10 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml
• Immunogen: His tagged bacterial fusion protein of caspase-10 p20
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with mAb anti-capsase 10 antibody. Proteins were visualized using a goat anti-rat secondary antibody conjugated to HRP and a chemiluminescence system.)

Related Product Information for anti-CASP10 antibody

There are two types of apoptotic caspases, initiator (apical) caspases and effector (executioner) caspases. Caspase 10 is one of the initiator caspases that cleave inactive pro-forms of effector caspases, thereby activating them. Four isoforms, namely Caspase-10a,-10 b,-10c,and -10d have been identified. They derived from a single chain polypeptide proenzyme but have different mature large and small subunits by cleavage. After the death ligand-receptor binding, caspade-10 has joined the multimeric Fas/TNF receptor complex and gets cleaved. The cleaved caspase-10 further acts on other effector caspases to initiate the caspase cascade.

Product Categories/Family for anti-CASP10 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 330864684
• NCBI GeneID: 843
• NCBI Accession #: NP_001193471.1
• NCBI GenBank Nucleotide #: NM_001206542.1
• UniProt Accession #: Q92851; Q68HC0; Q6KF62; Q6KF63; Q8IUP5; Q8WYQ8; Q99845; Q9Y2U6; Q9Y2U7
• Molecular Weight: 58,951 Da
• NCBI Official Full Name: caspase-10 isoform 5 preproprotein
• NCBI Official Synonym Full Names: caspase 10, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP10
• NCBI Official Synonym Symbols: MCH4; ALPS2; FLICE2
• NCBI Protein Information: caspase-10; CASP-10; FADD-like ICE2; apoptotic protease MCH-4; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; caspase 10, apoptosis-related cysteine protease; FAS-associated death domain protein interleukin-1B-converting enzyme 2
• UniProt Protein Name: Caspase-10
• Protein Family: Caspase
• UniProt Gene Name: CASP10
• UniProt Synonym Gene Names: MCH4; CASP-10; FLICE2
• UniProt Entry Name: CASPA_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Ref.12Isoform C is proteolytically inactive. Ref.12

Catalytic activity: Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Leu-Gln-Thr-Asp-|-Gly.

Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit

By similarity. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Ref.12

Tissue specificity: Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

Post-translational modification: Cleavage by granzyme B and autocatalytic activity generate the two active subunits.

Involvement in disease: Autoimmune lymphoproliferative syndrome 2A (ALPS2A) [MIM:603909]: A disorder of apoptosis that manifests in early childhood and results in the accumulation of autoreactive lymphocytes. It is characterized by non-malignant lymphadenopathy with hepatosplenomegaly, and autoimmune hemolytic anemia, thrombocytopenia and neutropenia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.14 Ref.17Familial non-Hodgkin lymphoma (NHL) [MIM:605027]: Cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.Note: The gene represented in this entry is involved in disease pathogenesis. Ref.15Gastric cancer (GASC) [MIM:613659]: A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.Note: The gene represented in this entry is involved in disease pathogenesis. Ref.16

Sequence similarities: Belongs to the peptidase C14A family.Contains 2 DED (death effector) domains.