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Activity (Sample Cell Assay Data: Activity was determined by the dose-dependent proliferation assay using a factor-dependent human erythroleukemic cell line TF-1 and was found to be less than 0.2ng/ml.)

EPO recombinant protein

EPO

Gene Names
EPO; EP; MVCD2
Applications
Cell Assay
Synonyms
EPO; Erythropoietin Recombinant Human Erythropoietin-Alpha (EPO); EPO recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Form/Format
Lyophilized from a 0.2 um filtered solution in PBS (pH7)
Applicable Applications for EPO recombinant protein
Cell Assay
Species
Human
Endotoxin Level
<1.0EU/ug of recombinant protein as determined by the LAL method.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: Activity was determined by the dose-dependent proliferation assay using a factor-dependent human erythroleukemic cell line TF-1 and was found to be less than 0.2ng/ml.)

Activity (Sample Cell Assay Data: Activity was determined by the dose-dependent proliferation assay using a factor-dependent human erythroleukemic cell line TF-1 and was found to be less than 0.2ng/ml.)

SDS-Page

(Purity: Recombinant Human EPO resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)

SDS-Page (Purity: Recombinant Human EPO resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)
Related Product Information for EPO recombinant protein
Recombinant Human EPO was expressed in E Coli cells. The protein accession number is P01588
EPO is predominantly synthesized and secreted by tubular and juxtatubular capillary, endothelial, and interstitial cells of the kidney. Approximately 10-15% of the total amount of EPO comes from extrarenal sources and is predominantly produced by hepatocytes and Kupffer cells of the liver. Approximately 40% of the molecular mass of EPO is due to its glycosylation. Glycosylation is an important factor determining the pharmacokinetic behaviour of EPO in vivo. Non-glycosylated Epo has an extremely short biological half life. Recombinant Human EPO is a glycosylated protein that runs at approximately 35kDa owing to its glycosylation.
Product Categories/Family for EPO recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,307 Da
NCBI Official Full Name
erythropoietin
NCBI Official Synonym Full Names
erythropoietin
NCBI Official Symbol
EPO
NCBI Official Synonym Symbols
EP; MVCD2
NCBI Protein Information
erythropoietin; epoetin
UniProt Protein Name
Erythropoietin
Protein Family
UniProt Gene Name
EPO
UniProt Entry Name
EPO_HUMAN

NCBI Description

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function: protein binding

Biological Process: erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of Ras protein signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2

Research Articles on EPO

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Product Notes

The EPO epo (Catalog #AAA516951) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EPO can be used in a range of immunoassay formats including, but not limited to, Cell Assay. Researchers should empirically determine the suitability of the EPO epo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPO, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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