Mouse anti-Human Carbonic Anhydrase VIII/CA8 Monoclonal Antibody | anti-CA8 antibody

Mouse anti Human Carbonic Anhydrase VIII/CA8 Monoclonal Antibody

Cat. Num. AAA2543395

• Gene Names: CA8; CALS; CARP; CA-RP; CAMRQ3; CA-VIII
• Reactivity: Human
• Applications: ELISA
• Synonyms: Carbonic Anhydrase VIII/CA8; Monoclonal Antibody; Mouse anti Human Carbonic Anhydrase VIII/CA8 Monoclonal Antibody; anti-CA8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Sequence Length: 290

• Applicable Applications for anti-CA8 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect Human CA8. The detection limit for Human CA8 is approximately 0.16 ng/well.
• Immunogen: Recombinant Human Carbonic Anhydrase VIII/CA8 protein
• Buffer: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase VIII/CA8 (rh Carbonic Anhydrase VIII/CA8; NP_004047.3; Met 1-Gln 290). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 80477937
• NCBI GeneID: 767
• Molecular Weight: 32,973 Da
• NCBI Official Full Name: Carbonic anhydrase VIII
• NCBI Official Synonym Full Names: carbonic anhydrase 8
• NCBI Official Symbol: CA8
• NCBI Official Synonym Symbols: CALS; CARP; CA-RP; CAMRQ3; CA-VIII
• NCBI Protein Information: carbonic anhydrase-related protein
• UniProt Protein Name: Carbonic anhydrase-related protein
• Protein Family: Carbonic anhydrase-related protein
• UniProt Gene Name: CA8
• UniProt Synonym Gene Names: CALS; CARP; CA-VIII
• UniProt Entry Name: CAH8_HUMAN

NCBI Description

The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

CA8: Does not have a carbonic anhydrase catalytic activity. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3). CMARQ3 is a congenital cerebellar ataxia associated with dysarthia, quadrupedal gait and mild mental retardation. Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 8q12.1

Molecular Function: protein binding

Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 3