Mouse anti-Human CAMKK1 / CaMKK Monoclonal Antibody | anti-CAMKK1 antibody

Mouse Monoclonal [clone 1F6] (IgG1,k) to Human CAMKK1 / CaMKK

Cat. Num. AAA246513

• Gene Names: CAMKK1; CAMKKA
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CAMKK1 / CaMKK; Monoclonal Antibody; Mouse Monoclonal [clone 1F6] (IgG1; k) to Human CAMKK1 / CaMKK; Anti-CAMKK1 / CaMKK Antibody (clone 1F6) IHC-plus; CAMKK1; CaM-kinase IV kinase; CaM-kinase kinase 1; CaM-kinase kinase alpha; CAMKK alpha protein; CAMKKA; CaM-KK 1; CaMKK; CaM-KK alpha; CaMKK 1; CaMKK alpha; CAMKKalpha; Human CAMKK1; anti-CAMKK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1F6
• Specificity: Human CaMKK
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 505

• Applicable Applications for anti-CAMKK1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: CAMKK1 (AAH31647, 1 a.a. ~ 521 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: CAMKK1 / CaMKK antibody was raised against cAMKK1 (AAH31647, 1 a.a. ~ 521 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CAMKK1 / CaMKK antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody, clone 1F6.)

Western Blot (WB)

(Western blot of CAMKK1 expression in Jurkat cells by CAMKK1 monoclonal antibody, clone 1F6.)

RNA interference

(Western blot of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMKK1 monoclonal antibody clone 1F6. GAPDH (36.1 kD) used as control.)

ELISA (EIA)

(Detection limit for recombinant GST tagged CAMKK1 is approximately 1 ng/ml as a capture antibody.)

Related Product Information for anti-CAMKK1 antibody

CAMKK1 belongs to the CAMKK subfamily of Serine/Threonine Kinases. CAMKK1 regulates the calcium/calmodulin-dependent protein kinase cascade by phosphorylating CAMKI, CAMKIV, and PKB.

Product Categories/Family for anti-CAMKK1 antibody

Family: Protein Kinase; Subfamily: CAMKK

NCBI and Uniprot Product Information

• NCBI GI #: 14150045
• NCBI GeneID: 84254
• NCBI Accession #: NP_115670.1
• NCBI GenBank Nucleotide #: NM_032294.2
• UniProt Accession #: Q8N5S9; Q9BQH3
• Molecular Weight: 57,523 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase kinase 1 isoform a
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase kinase 1, alpha
• NCBI Official Symbol: CAMKK1
• NCBI Official Synonym Symbols: CAMKKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase kinase 1; caMKK 1; caM-KK 1; caM-KK alpha; CAMKK alpha protein; caM-kinase kinase 1; caM-kinase IV kinase; caM-kinase kinase alpha; calcium/calmodulin-dependent protein kinase kinase alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase kinase 1
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMKK1
• UniProt Synonym Gene Names: CAMKKA; CaM-KK 1; CaM-kinase kinase 1; CaMKK 1; CaM-KK alpha; CaM-kinase kinase alpha; CaMKK alpha
• UniProt Entry Name: KKCC1_HUMAN

NCBI Description

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMKK1: a calcium/calmodulin-dependent S/T protein kinase of the CAMKK family. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death. 2 isoforms of the human protein are produced by alternative splicing. The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition. Binding of calmodulin may releave intrasteric autoinhibition. May be regulated through phosphorylation by CAMK1 and CAMK4. Appears to be autophosphorylated in a Ca2+/calmodulin-dependent manner. Phosphorylation of Ser-458 is blocked upon binding to Ca2+/calmodulin.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; EC 2.7.11.17; Other group; CAMKK family; Meta subfamily

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: nucleus; cytosol

Molecular Function: calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: synaptic transmission; protein amino acid phosphorylation