Mouse anti-Human CAMK1D Monoclonal Antibody | anti-CAMK1D antibody

Mouse Monoclonal [clone 3H8] (IgG2b,k) to Human CAMK1D

Cat. Num. AAA246388

• Gene Names: CAMK1D; CKLiK; CaM-K1; CaMKID
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CAMK1D; Monoclonal Antibody; Mouse Monoclonal [clone 3H8] (IgG2b; k) to Human CAMK1D; Anti-CAMK1D Antibody (clone 3H8) IHC-plus; CaM kinase ID; CaMKI-like protein kinase; CaMKID; CKLiK; CaM kinase I delta; CaM-K1; CaMKI delta; CaM-KI delta; CaMKI-like kinase; Human CAMK1D; anti-CAMK1D antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3H8
• Specificity: Human CAMK1D
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 357

• Applicable Applications for anti-CAMK1D antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using tissue lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: CAMK1D (AAH35745, 1 a.a. ~ 96 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: CAMK1D antibody was raised against cAMK1D (AAH35745, 1 a.a. ~ 96 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CAMK1D antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of CAMK1D expression in transfected 293T cell line by antibody.)

ELISA (EIA)

(Detection limit for recombinant GST tagged CAMK1D is 0.3 ng/ml as a capture antibody.)

Related Product Information for anti-CAMK1D antibody

CAMK1-Like kinase belongs to the CAMK1 subfamily of Serine/Threonine Kinases. CAMK1-Like kinase is a polymorphonuclear leukocyte - specific kinase that mediates the regulation of granulocyte functions.

Product Categories/Family for anti-CAMK1D antibody

Family: Protein Kinase; Subfamily: CAMK1

NCBI and Uniprot Product Information

• NCBI GI #: 9966875
• NCBI GeneID: 57118
• NCBI Accession #: NP_065130.1
• NCBI GenBank Nucleotide #: NM_020397.2
• UniProt Accession #: Q8IU85; Q9HD31; B0YIY0
• Molecular Weight: 40,190 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type 1D isoform 1
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase ID
• NCBI Official Symbol: CAMK1D
• NCBI Official Synonym Symbols: CKLiK; CaM-K1; CaMKID
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type 1D; caMKI delta; caM-KI delta; CaM kinase ID; caM kinase I delta; CamKI-like protein kinase
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type 1D
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK1D
• UniProt Synonym Gene Names: CAMKID; CaM kinase ID; CaM-KI delta; CaMKI delta; CaMKID; CKLiK
• UniProt Entry Name: KCC1D_HUMAN

NCBI Description

This gene encodes a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. The encoded protein may be involved in the regulation of granulocyte function through the chemokine signal transduction pathway. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK1D: a calcium/calmodulin-dependent S/T protein kinase of the CAMK group. Binding of calmodulin is thought to result in a conformational change and can lead to subsequent activation through phosphorylation by CAMKK1 and CAMKK2. Can be activated by CAMKK1 in a calcium-independent manner. Its Ca(2+)/CaM-dependent activity is enhanced 30-fold in vitro by phosphorylation at Thr180 by CaMKK1. Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1. Expression increases upon treatment of EC cells with DMSO and retinoic acid. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Kinase, protein; EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: cytoplasm; nucleus

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: positive regulation of apoptosis; positive regulation of phagocytosis; activation of CREB transcription factor; regulation of dendrite development; inflammatory response; protein amino acid phosphorylation; negative regulation of apoptosis