Mouse CaM Kinase II, alpha Monoclonal Antibody | anti-CAMK2A antibody

CaM Kinase II, alpha (Calmodulin-Dependent Protein Kinase II)

Cat. Num. AAA632044

• Gene Names: CAMK2A; CAMKA; KIAA0968
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CaM Kinase II; alpha; Monoclonal Antibody; alpha (Calmodulin-Dependent Protein Kinase II); Anti -CaM Kinase II; anti-CAMK2A antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3G55
• Specificity: Recognizes the alpha subunit of rat CaM Kinase II. No signi cant crossreactivity was seen with the Beta, Beta1, gammaB, epsilonA, epsilonB and epsilonC isoforms. The epitope is located in the regulatory domain. Species Crossreactivity: human, mouse and gold sh.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-CAMK2A antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot.; Dilution: ELISA: 0.1-1ug/ml; Immunohistochemistry (Paraf n): 1-5ug/ml; Immunoprecipitation/IP Kinase Assays: 2ug; Western Blot: 1ug/ml
• Immunogen: Puri?ed rat brain CaM Kinase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CAMK2A antibody

CaM Kinase II (Ca 2+ /calmodulin-dependent protein kinase II) is a multifunctional Ca 2+ /calmodulin-dependent serine/threonine kinase which is able to phosphorylate a variety of proteins in response to Ca 2+ signals. This kinase participates in the regulation of numerous cellular functions including: 1) metabolism of carbohydrates, lipids, and amino acids, 2) neurotransmitter synthesis and release, 3) regulation of ion ux through ion channels, 4) Ca 2+ homeostasis, 5) cytoskeletal function, and 6) Ca 2+ -regulated changes in gene expression. CaM kinase II is broadly expressed and is highly concentrated in brain particularly in cortical structure and hippocampus. This kinase also plays an essential role in induction of long-term potentiation. Puri ed rat brain CaM kinase has an apparent molecular weight of ~540kD and consists of isozymes composed of various ratios of 54kD (a), 58kD (b'), 60kD (b), 59kD (g) and 60kD (d) in a holoenzyme containing 10-12 subunits. The primary difference in the subunit isoforms lies in the variable region located at the carboxyl-end of the calmodulin binding domain. Ca 2+ /calmodulin-stimulated autophosphorylation of CaM kinase II occurs on multiple residues. In particular, autophosphorylation on residue Thr 286 /Thr 287 within the regulatory domain results in full activation of the enzyme, and in the generation of Ca 2+ /calmodulin-independent activity. Autophosphorylation has also been reported to increase the af nity of CaM kinase for Ca 2+ /calmodulin.

Product Categories/Family for anti-CAMK2A antibody

Antibodies; Abs to Calmodulin

NCBI and Uniprot Product Information

• NCBI GI #: 119582146
• NCBI GeneID: 815
• UniProt Accession #: Q7LDD5; Q8IWE0; Q9UQM7
• Molecular Weight: 54,088 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, isoform CRA_b
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: CAMK2A
• NCBI Official Synonym Symbols: CAMKA; KIAA0968
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha; CaMKIINalpha; OTTHUMP00000165787; OTTHUMP00000165788; CaMK-II alpha subunit; caMK-II subunit alpha; CaM-kinase II alpha chain; CaM kinase II alpha subunit; caM kinase II subunit alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, isoform CRA_a
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2A
• UniProt Entry Name: Q7LDD5_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: presynaptic membrane; nucleoplasm; mitochondrion; plasma membrane; cytosol; nucleus; cell junction

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; glutamate receptor binding; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; calcium ion transport; cytokine and chemokine mediated signaling pathway; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; regulation of neuronal synaptic plasticity; activation of NF-kappaB transcription factor; G1/S transition of mitotic cell cycle