Mouse Calsequestrin Monoclonal Antibody | anti-CASQ2 antibody

Mouse Anti Human Calsequestrin

Cat. Num. AAA140400

• Gene Names: CASQ2; PDIB2
• Applications: ELISA, Western Blot
• Purity: CASQ2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Calsequestrin; Monoclonal Antibody; Mouse Anti Human Calsequestrin; CASQ2 Antibody; Calsequestrin-2; Mouse Anti Human; PDIB2; CASQ2; Calsequestrin cardiac muscle isoform; FLJ26321; FLJ93514; anti-CASQ2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and k light chain
• Clone Number: PAT4E10AT
• Purity/Purification: CASQ2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 399

• Applicable Applications for anti-CASQ2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: CASQ2 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000.Recommended starting dilution is 1:1000.
• Immunogen: Anti-human CASQ2 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CASQ2 amino acids 20-399 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-CASQ2 antibody

Introduction: CASQ2 belongs to the calsequestrin family and is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. CASQ2 is a calcium binding protein that stores calcium for muscle function. The discharge of calcium bound to CASQ2 through a calcium release channel activates muscle contraction. CASQ2 binds 40 to 50 moles of calcium. CASQ2 mutations result in stress-induced polymorphic ventricular tachycardia, also called catecholaminergic polymorphic ventricular tachycardia 2 which is known fir its bidirectional ventricular tachycardia that causes cardiac arrest.

Product Categories/Family for anti-CASQ2 antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 119395727
• NCBI GeneID: 845
• NCBI Accession #: NP_001223.2
• NCBI GenBank Nucleotide #: NM_001232.3
• Molecular Weight: 38,269 Da
• NCBI Official Full Name: calsequestrin-2
• NCBI Official Synonym Full Names: calsequestrin 2 (cardiac muscle)
• NCBI Official Symbol: CASQ2
• NCBI Official Synonym Symbols: PDIB2
• NCBI Protein Information: calsequestrin-2; calsequestrin 2, fast-twitch, cardiac muscle; calsequestrin, cardiac muscle isoform
• UniProt Protein Name: Calsequestrin-2
• Protein Family: Calsequestrin
• UniProt Gene Name: CASQ2
• UniProt Entry Name: CASQ2_HUMAN

NCBI Description

The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq, Jul 2008]

Uniprot Description

calsequestrin 2: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+) ions. Defects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2); also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. Belongs to the calsequestrin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: sarcoplasmic reticulum membrane; sarcoplasmic reticulum lumen; sarcoplasmic reticulum; junctional membrane complex; cytoplasm; voltage-gated calcium channel complex; intracellular; Z disc

Molecular Function: protein binding; protein homodimerization activity; calcium ion binding; calcium-dependent protein binding

Biological Process: protein polymerization; striated muscle contraction; regulation of heart rate; negative regulation of potassium ion transport; sequestering of calcium ion; sarcomere organization; detection of calcium ion; transmembrane transport; cardiac muscle contraction

Disease: Ventricular Tachycardia, Catecholaminergic Polymorphic, 1, With Or Without Atrial Dysfunction And/or Dilated Cardiomyopathy; Ventricular Tachycardia, Catecholaminergic Polymorphic, 2