Mouse anti-Bovine Calpain Small Subunit Monoclonal Antibody | anti-Capns1 antibody
Calpain Small Subunit (Mu Calpain)
Gene Names
Capns1; Capn4
Reactivity
Bovine
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Purity
AscitesAscites
Synonyms
Calpain Small Subunit; Monoclonal Antibody; Calpain Small Subunit (Mu Calpain); Anti -Calpain Small Subunit (Mu Calpain); anti-Capns1 antibody
Host
Mouse
Reactivity
Bovine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q30
Specificity
Reacts with the small subunit of human erthrocyte u-calpain or bovine myocardial m-calpain. Does not react with calpain large subunits
Purity/Purification
Ascites
Ascites
Ascites
Form/Format
Supplied as a liquid. No preservative added.
Applicable Applications for anti-Capns1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation.
Dilution: Western Blot: 1:500-1000. Recognizes the 30kD small subunits of either calpain isozyme.
ELISA: 1:50,000
Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue.
Immunocytochemistry: 1:100
Immunoprecipitation: (small unit, in the presence of calcium large & small complex).
Dilution: Western Blot: 1:500-1000. Recognizes the 30kD small subunits of either calpain isozyme.
ELISA: 1:50,000
Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue.
Immunocytochemistry: 1:100
Immunoprecipitation: (small unit, in the presence of calcium large & small complex).
Immunogen
Bovine myocardial mu-calpain
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Capns1 antibody
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I Calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
Product Categories/Family for anti-Capns1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,570 Da
NCBI Official Full Name
calpain small subunit
NCBI Official Synonym Full Names
calpain, small subunit 1
NCBI Official Symbol
Capns1
NCBI Official Synonym Symbols
Capn4
NCBI Protein Information
calpain small subunit 1; CDPS; CSS1; calpain 4; CANP small subunit; calpain regulatory subunit; calcium-dependent protease small subunit 1; calcium-activated neutral proteinase small subunit
UniProt Protein Name
Calpain small subunit 1
Protein Family
UniProt Gene Name
Capns1
UniProt Synonym Gene Names
Capn4; Css1
UniProt Entry Name
CPNS1_RAT
NCBI Description
small subunit of calcium-activated protease calpain; functions only in conjunction with large subunit [RGD]
Uniprot Description
CAPNS1: Regulatory subunit of the calcium-regulated non- lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Protein type: Calcium-binding; Protease; Motility/polarity/chemotaxis
Cellular Component: cytoplasm; plasma membrane
Molecular Function: protein heterodimerization activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding
Biological Process: proteolysis
Research Articles on Capns1
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Product Notes
The Capns1 capns1 (Catalog #AAA602020) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Calpain Small Subunit (Mu Calpain) reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Calpain Small Subunit can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation. Dilution: Western Blot: 1:500-1000. Recognizes the 30kD small subunits of either calpain isozyme. ELISA: 1:50,000 Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100 Immunoprecipitation: (small unit, in the presence of calcium large & small complex). Researchers should empirically determine the suitability of the Capns1 capns1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calpain Small Subunit, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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