Mouse Calcium and Integrin Binding Protein 1 Monoclonal Antibody | anti-CIB1 antibody

Calcium and Integrin Binding Protein 1 (SIP2-28, Calmyrin)

Cat. Num. AAA603807

• Gene Names: CIB1; CIB; KIP; KIP1; SIP2-28
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Calcium and Integrin Binding Protein 1; Monoclonal Antibody; Calcium and Integrin Binding Protein 1 (SIP2-28; Calmyrin); Anti -Calcium and Integrin Binding Protein 1 (SIP2-28; anti-CIB1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 8k268
• Specificity: Recognizes human Calcium and Integrin Binding Protein 1 (SIP2-28, Calmyrin).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-CIB1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1000-1:2,000; Western Blot: 1:1000-1:2,000
• Immunogen: Recombinant human CIB1 (1-191aa) purified from E. coli.
• Hybridoma: Mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CIB1 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CIB1 antibody

CIB1 (Calcium and integrin binding 1) is regulatory protein with 50% homology to calmodulin and calcineurin B, that encodes a member of the calcium-binding protein family. The specific function of this protein has not yet been determined; however this protein is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining.

Product Categories/Family for anti-CIB1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 163644313
• NCBI GeneID: 10519
• NCBI Accession #: NP_006375.2
• NCBI GenBank Nucleotide #: NM_006384.3
• UniProt Accession #: Q99828; O00693; O00735; Q6IB49; Q96J54; Q99971; B5BU40
• Molecular Weight: 21,703 Da
• NCBI Official Full Name: calcium and integrin-binding protein 1
• NCBI Official Synonym Full Names: calcium and integrin binding 1 (calmyrin)
• NCBI Official Symbol: CIB1
• NCBI Official Synonym Symbols: CIB; KIP; KIP1; SIP2-28
• NCBI Protein Information: calcium and integrin-binding protein 1; CIBP; calmyrin; OTTHUMP00000194684; kinase-interacting protein; DNA-PKcs-interacting protein; SNK-interacting protein 2-28; Snk interacting protein 2-28; calcium and integrin binding protein; calcium- and integrin-binding protein; DNA-dependent protein kinase interacting protein; calcium and integrin binding, protein kinase interacting protein
• UniProt Protein Name: Calcium and integrin-binding protein 1
• Protein Family: Calcium and integrin-binding protein
• UniProt Gene Name: CIB1
• UniProt Synonym Gene Names: CIB; KIP; PRKDCIP
• UniProt Entry Name: CIB1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the calcium-binding protein family. The specific function of this protein has not yet been determined; however this protein is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining. This protein also interacts with integrin alpha(IIb)beta(3), which may implicate this protein as a regulatory molecule for alpha(IIb)beta(3). [provided by RefSeq]

Uniprot Description

CIB1: May convert the inactive conformation of integrin alpha- IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2/FAK1 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Participates in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation.

Protein type: Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q25.3-q26

Cellular Component: Golgi apparatus; filopodium tip; centrosome; neuron projection; endoplasmic reticulum; nucleoplasm; growth cone; cell soma; membrane; perinuclear region of cytoplasm; lamellipodium; cytoplasm; apical plasma membrane; plasma membrane; nucleus; sarcolemma; vesicle

Molecular Function: protein binding; Ras GTPase binding; protein anchor; calcium ion binding

Biological Process: apoptosis; endomitotic cell cycle; negative regulation of megakaryocyte differentiation; cytoplasmic microtubule organization and biogenesis; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; double-strand break repair; positive regulation of cell proliferation; angiogenesis; cell adhesion; positive regulation of cell-matrix adhesion; negative regulation of protein kinase B signaling cascade; negative regulation of microtubule depolymerization; positive regulation of cell adhesion mediated by integrin; positive regulation of metalloenzyme activity; positive regulation of cell growth; regulation of cell proliferation; cell division; platelet formation; positive regulation of protein amino acid phosphorylation; response to DNA damage stimulus; regulation of cell division; spermatid development; positive regulation of cell migration; negative regulation of apoptosis