Rat anti-Mouse C5aR Monoclonal Antibody | anti-C5aR antibody
C5aR, Mouse, mAb 20/70
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Application Use: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of mouse C5aR to be inactivated.
3. Godau, J et al; C5a initiates the inflammatory cascade in immune complex peritonitis. J Immunol 2004, 173: 3437
4. Chen, NJ et al; C5L2 is critical for the biological activities of the anaphylatoxins C5a and C3a. Nature 2007, 446: 203
5. Rittirsch, D et al; Functional roles for C5a receptors in sepsis. Nat Med 2008, 14: 551
6. Gueler, F et al; Complement 5a Receptor inhibition improves renal allograft survival. J Am Soc Nephrol 2008, 19: 2302
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Product Notes
The C5aR (Catalog #AAA584113) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The C5aR, Mouse, mAb 20/70 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's C5aR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), FS, Immunofluorescence (IF). Application Notes: FC: 2x105 cells were blocked with human and murine IgG on ice for 20';. Cells were stained with 10 ug antibody20/70 for 45'; in PBS/1.5%FCS. For analysis with FACS cells were washed with PBS/1%formaldehyde (Ref.2). Application Use: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of mouse C5aR to be inactivated. Researchers should empirically determine the suitability of the C5aR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C5aR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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