Mouse C5aR Monoclonal Antibody | anti-C5aR antibody

C5aR, Human, mAb S5/1

Cat. Num. AAA584430

• Reactivity: Reactivity: Human. Cross reactivity: Rabbit
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: C5aR; Monoclonal Antibody; Human; mAb S5/1; Complement component 5a receptor 1; CD88; C5a anaphylatoxin receptor.; anti-C5aR antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Rabbit
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-C5aR antibody: F, Flow Cytometry (FC/FACS), FS, P, Western Blot (WB)
• Application Notes: Application Notes: FC: Antibody S5/1 stains the N-terminal domain of CD88. 1x106 leukocytes were incubated with 2ug of antibody (Ref. 1).; Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of binding of C5a to its receptor dilutions have to be made according to the amounts of C5a to be inhibited.
• Immunogen: Synthetic peptide comprising the N-terminal domain of the C5aR (Met1-Asn31)
• Positive Control: Whole blood granulocytes
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-C5aR antibody

Product Description: The monoclonal antibody S5/1 recognizes the human receptor for C5a (CD88). The C5a receptor- is a seven transmembrane GTP-binding-protein-coupled receptor of 45kDa which binds with high affinity to human anaphylatoxin C5a. Upon C5a binding- G-protein-dependent cellular- responses are initiated such as increased intracellular Ca2+, granule fusion with the cell membrane, enzyme release and oxidative burst suggesting that C5aR is an important mediator of inflammation. The C5a receptor is expressed on a broad range of cell types, including- neutrophils, monocytes/macrophages, dendritic cells, astrocytes and microglia.
Interestingly, C5a receptor signalling has been associated with several inflammatory diseases including rheumatoid arthritis.-
Clone S5/1 was raised against a synthetic peptide comprising the N-terminal domain of the C5aR (Met1-Asn31). The antibody has been shown to inhibit the binding of C5a to its receptor.

References

1. Opperman, M et al; Probing the human receptor for C5a anaphylatoxin with side-directed antibodies. J Immunol 1993, 151: 3785 2. Opperman, M et al; Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors. Immunol. 1994, 82: 516
3. Werfel, T et al; C5a receptors are detectable on mast cells in normal human skin and in psoriatic plaques but not in weal and flare reactions or in urticaria pigmentosa by immunohistochemistry. Arch Dermatol Res 1997, 289: 83
4. Zwirner, J et al; Evaluation of C3a receptor expression on human leucocytes by the use of novel monoclonal antibodies. Immunology 1999, 97: 166
5. Fortin, J et al; Contractile effect of anaphylatoxin C5a and of a mimetic peptide on the human umbilical artery: further evidence for leukocyte-dependent vasomotion. J Cardiovasc Pharmacol. 2002, 40:815
6. Gueler, F et al; Complement 5a receptor inhibition improves renal allograft survival. J Am Soc Nephrol 2008, 19: 2302
7. Strey, CW et al;. Complement and neutrophil function changes after liver resection in humans. World J Surg 2009, 33: 2635
8. Werfel, T et al: CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin. J Immunol 1996, 157: 1729.
9. Petitclerc, E et al: Pathologic leukocyte infiltration of the rabbit aorta confers a vasomotor effect to chemotactic peptides through cyclooxygenase-derived metabolites. J Immunol 1996, 156: 3426