Rat anti-Mouse C5aR Monoclonal Antibody | anti-C5aR antibody
C5aR, Mouse, mAb 10/92
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Synonyms
C5aR; Monoclonal Antibody; Mouse; mAb 10/92; C5aR1; CD88; anti-C5aR antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-C5aR antibody
F, Flow Cytometry (FC/FACS), Western Blot (WB
Application Notes
Application Use: For immunohistology, Western-blotting-and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Immunogen
RBL-2H3 transfectants expressing murine C5aR
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-C5aR antibody
Product Description: The monoclonal antibody 10/92 recognizes the mouse receptor C5a (C5aR, CD88). The mouse anaphylatoxin C5a is a 74-residu glycopolypeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a could be demonstrated, rendering this molecule an important mediator of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP-binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The 45 kDa C5a receptor is expressed by neutrophils, monocytes/macrophages, dendritic cells, astrocytes and microglia. In contrast, C5a receptor is not detectable on resting or stimulated murine T and B lymphocytes. The monoclonal antibody 10/92 does not inhibit the binding of C5a to its receptor.
References
Soruri, A et al; Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody. Immunol Lett 2003, 88: 47
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Product Notes
The C5aR (Catalog #AAA584416) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The C5aR, Mouse, mAb 10/92 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's C5aR can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), Western Blot (WB. Application Use: For immunohistology, Western-blotting-and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the C5aR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C5aR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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