Rabbit anti-Human RNT2 Polyclonal Antibody | anti-RNT2 antibody

RNT2, NT (RNASET2, RNASE6PL, Ribonuclease T2, Ribonuclease 6) (Azide free) (HRP)

Cat. Num. AAA6343043

• Gene Names: RNASET2; RNASE6PL; bA514O12.3; RP11-514O12.3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RNT2; Polyclonal Antibody; NT (RNASET2; RNASE6PL; Ribonuclease T2; Ribonuclease 6) (Azide free) (HRP); RNASET2; Ribonuclease 6; anti-RNT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RNT2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RNT2 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 25-55 in the N-terminal region of human RNT2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NCI-H460 cell line lysates (35ug/lane) usingMBS643112. This demonstrates MBS643112 detected the RNT2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of RNT2 in formalin fixed and paraffin embedded human spleen carcinoma using MBS643112 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-RNT2 antibody

Antibodies; Enzymes; Ribonuclease

References

1. Fransen, K., et al. Hum. Mol. Genet. 19(17):3482-3488(2010). 2. Quan, C., et al. Nat. Genet. 42(7):614-618(2010). 3. Liu, J., et al. J. Cell. Mol. Med. 14(5):1146-1155(2010). 4. Henneke, M., et al. Nat. Genet. 41(7):773-775(2009). 5. Monti, L., et al. Oncol. Res. 17(2):69-74(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5231228
• NCBI GeneID: 8635
• NCBI Accession #: NP_003721.2
• NCBI GenBank Nucleotide #: NM_003730.4
• UniProt Accession #: O00584; Q5T8Q0; Q8TCU2; Q9BZ46; Q9BZ47; B2RDA7; E1P5C3
• Molecular Weight: 29,481 Da
• NCBI Official Full Name: ribonuclease T2
• NCBI Official Synonym Full Names: ribonuclease T2
• NCBI Official Symbol: RNASET2
• NCBI Official Synonym Symbols: RNASE6PL; bA514O12.3; RP11-514O12.3
• NCBI Protein Information: ribonuclease T2; ribonuclease 6
• UniProt Protein Name: Ribonuclease T2
• UniProt Gene Name: RNASET2
• UniProt Synonym Gene Names: RNASE6PL
• UniProt Entry Name: RNT2_HUMAN

NCBI Description

This ribonuclease gene is a novel member of the Rh/T2/S-glycoprotein class of extracellular ribonucleases. It is a single copy gene that maps to 6q27, a region associated with human malignancies and chromosomal rearrangement. [provided by RefSeq, Jul 2008]

Uniprot Description

RNASET2: Has ribonuclease activity, with higher activity at acidic pH. May play a role in cellular RNA catabolism. Defects in RNASET2 are the cause of leukoencephalopathy cystic without megalencephaly (LCWM). An infantile- onset syndrome of cerebral leukoencephalopathy. Affected newborns develop microcephaly and neurologic abnormalities including psychomotor impairment, seizures and sensorineural hearing impairment. The brain shows multifocal white matter lesions, anterior temporal lobe subcortical cysts, pericystic abnormal myelination, ventriculomegaly and intracranial calcifications. Belongs to the RNase T2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.1.27.-; Secreted; RNA-binding; Ribonuclease

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: lysosomal lumen; extracellular space; endoplasmic reticulum lumen; lysosome; extracellular region

Molecular Function: ribonuclease T2 activity; RNA binding; ribonuclease activity

Biological Process: RNA catabolic process

Disease: Leukoencephalopathy, Cystic, Without Megalencephaly