Rabbit anti-Human C5  Monoclonal Antibody | anti-C5  antibody

Rabbit anti Human C5  Monoclonal Antibody

Cat. Num. AAA2544041

• Gene Names: C5; C5D; C5a; C5b; ECLZB; CPAMD4
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: C5 ; Monoclonal Antibody; Rabbit anti Human C5 Monoclonal Antibody; anti-C5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 1

• Applicable Applications for anti-C5 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: IHC-P: 1-10 mug/mL
• Immunogen: Recombinant Human Complement C5a protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-C5  antibody

C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 alpha-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.

NCBI and Uniprot Product Information

• NCBI GI #: 30023536
• NCBI GeneID: 727
• Molecular Weight: 188,305 Da
• NCBI Official Full Name: C5, partial
• NCBI Official Synonym Full Names: complement C5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: C5D; C5a; C5b; ECLZB; CPAMD4
• NCBI Protein Information: complement C5
• UniProt Protein Name: Complement C5
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: CPAMD4
• UniProt Entry Name: CO5_HUMAN

NCBI Description

This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q33-q34

Cellular Component: extracellular region

Molecular Function: chemokine activity; protein binding; receptor binding

Biological Process: activation of MAPK activity; cell surface receptor linked signal transduction; chemotaxis; complement activation; G-protein coupled receptor protein signaling pathway; regulation of complement activation; response to stress

Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To