Mouse anti-Human, Rat C1q Receptor, gamma Monoclonal Antibody | anti-C1QC antibody

C1q Receptor, gamma (gC1qR, C1qBP, Complement Component 1, Q Subcomponent-binding Protein)

Cat. Num. AAA646522

• Gene Names: C1QC; C1QG; C1Q-C
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: C1q Receptor; gamma; Monoclonal Antibody; gamma (gC1qR; C1qBP; Complement Component 1; Q Subcomponent-binding Protein); Anti -C1q Receptor; anti-C1QC antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 13A19
• Specificity: Recognizes epitopes in the XC15 peptide that contains a binding site for high-molecular-weight kininogen and Factor XII. This clone recognizes both the mature (aa74-282) and truncated form, lacking residues 74-95. Species Crossreactivity: rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA.

• Applicable Applications for anti-C1QC antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, Functional Studies, ELISA, Immunofluorescence and Immunoprecipitation.; Dilution: Flow Cytometry: 1:50
• Immunogen: Recombinant corresponding to aa74-282 from mature gC1qR.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-C1QC antibody

This monoclonal antibody recognizes a cell membrane C1q binding molecule that recognizes the globular heads of C1q. It is also present in plasma and the extracellular matrix. The molecule is an unusually acidic, single chain protein with an apparent molecular weight of 33kD. It does not possess a conventional sequence motif compatible with a membrane spanning segment nor a consensus site for a GPI anchor. gC1qR migrates as a single chain under both reducing and non-reducing conditions, but it behaves as an oligomer on gel-filtration in non-dissociating conditions. Its multimer formation may be a critical process by which the gC1qR molecule increases its affinity for multivalent ligands such as C1q. gC1qR has been shown to inhibit complement activation by preventing the binding of C1q to antibodies, suggesting that the binding site for gC1qR and the binding site for immune complexes, which are present on the C1q globular 'heads', may be located at the same position. gC1qR is capable of interacting with several proteins involved in blood clotting, namely, thrombin, prothrombin, the heparinbinding form of vitronectin, the ternary complex, vitronectin-thrombin-antithrombin, as well as high-molecular-weight kininogen and Hageman factor. Besides its role in the complement pathway, gC1qR participates in enhancement of Fc-receptor and CR1-mediated phagocytosis, procoagulant activity on platelets, and chemotactic activity on mast cells, eosinophils, neutrophils, and fibroblasts. gC1qR is expressed on a wide variety of cells and can serve as a binding site for plasma and microbial proteins. Its antigenic sites may be cryptic on cells in suspension but become exposed when the cells are fixed by bifunctional cross-linkers. Probably it is also expressed on the cell membrane as a tetramer. Crosslinking or activation may thus bring about a tetrameric assembly of gC1qR followed by a conformational change within the molecule, thereby exposing epitopes which are otherwise hidden. A form of gC1qR is also found inside the cell. Intracellular gC1qR has been shown to bind the cytoplasmic tail of the a1B-adrenergic receptor and to PKCu.

Product Categories/Family for anti-C1QC antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 166235903
• NCBI GeneID: 714
• NCBI Accession #: NP_001107573.1
• NCBI GenBank Nucleotide #: NM_001114101.1
• UniProt Accession #: P02747; Q7Z502; Q96DL2; Q96H05
• Molecular Weight: 25,774 Da
• NCBI Official Full Name: complement C1q subcomponent subunit C
• NCBI Official Synonym Full Names: complement component 1, q subcomponent, C chain
• NCBI Official Symbol: C1QC
• NCBI Official Synonym Symbols: C1QG; C1Q-C
• NCBI Protein Information: complement C1q subcomponent subunit C; complement component 1, q subcomponent, gamma polypeptide
• UniProt Protein Name: Complement C1q subcomponent subunit C
• Protein Family: Complement C1q subcomponent
• UniProt Gene Name: C1QC
• UniProt Synonym Gene Names: C1QG
• UniProt Entry Name: C1QC_HUMAN

NCBI Description

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. A deficiency in C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N-terminus, and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the C-chain polypeptide of human complement subcomponent C1q. Alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Subunit structure: C1 is a calcium-dependent trimolecular complex of C1q, R and S in the molar ration of 1:2:2. C1q subcomponent is composed of nine subunits, six of which are disulfide-linked dimers of the A and B chains, and three of which are disulfide-linked dimers of the C chain.

Subcellular location: Secreted.

Post-translational modification: O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.

Involvement in disease: Complement component C1q deficiency (C1QD) [MIM:613652]: A disorder caused by impaired activation of the complement classical pathway. It generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9

Sequence similarities: Contains 1 C1q domain.Contains 1 collagen-like domain.