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Quality Control (Western blot analysis of immunized recombinant protein, using anti-BXDC1 monoclonal antibody. )

Mouse anti-Human BXDC1 Monoclonal Antibody | anti-BXDC1 antibody

Mouse monoclonal antibody Anti-Human BXDC1

Gene Names
RPF2; BXDC1; bA397G5.4
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Western Blot
Synonyms
BXDC1; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human BXDC1; Homo sapiens brix domain containing 1 (BXDC1); mRNA; RPF2; FLJ21087; bA397G5.4; anti-BXDC1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2099C7a
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
3704
Applicable Applications for anti-BXDC1 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-BXDC1 monoclonal antibody. )

Quality Control (Western blot analysis of immunized recombinant protein, using anti-BXDC1 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of human BXDC1 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2099C7a-1]Predicted molecular weight: 35 kDa)

Western Blot (WB) (Detection of human BXDC1 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2099C7a-1]Predicted molecular weight: 35 kDa)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BXDC1 mAb. Nuclear was stained with Hoechst (blue fluorescence). [Lot No. 2099C7a-1])

Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BXDC1 mAb. Nuclear was stained with Hoechst (blue fluorescence). [Lot No. 2099C7a-1])

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,583 Da
NCBI Official Full Name
Homo sapiens ribosome production factor 2 homolog (S. cerevisiae) (RPF2), transcript variant 1, mRNA
NCBI Official Synonym Full Names
ribosome production factor 2 homolog (S. cerevisiae)
NCBI Official Symbol
RPF2
NCBI Official Synonym Symbols
BXDC1; bA397G5.4
NCBI Protein Information
ribosome production factor 2 homolog; brix domain containing 1; brix domain-containing protein 1; homolog of Rpf2; ribosomal processing factor 2 homolog; ribosome biogenesis protein RPF2 homolog
UniProt Protein Name
Ribosome production factor 2 homolog
UniProt Gene Name
RPF2
UniProt Entry Name
RPF2_HUMAN

Uniprot Description

BXDC1: Belongs to the RPF2 family.

Protein type: Nucleolus; Unknown function

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleolus; nucleus

Molecular Function: rRNA binding

Biological Process: ribosomal large subunit assembly and maintenance; maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Research Articles on BXDC1

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Product Notes

The BXDC1 rpf2 (Catalog #AAA120116) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human BXDC1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BXDC1 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB). Researchers should empirically determine the suitability of the BXDC1 rpf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BXDC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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