Western Blot (WB)
(Western Blot analysis using immunogen as sample.)
Mouse anti-Human Brix Domain Containing 1 Monoclonal Antibody | anti-RPF2 antibody
Brix Domain Containing 1 (BXDC1, bA397G5.4, FLJ21087, RPF2)
Gene Names
RPF2; BXDC1; FLJ21087; bA397G5.4
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Brix Domain Containing 1; Monoclonal Antibody; Brix Domain Containing 1 (BXDC1; bA397G5.4; FLJ21087; RPF2); Anti -Brix Domain Containing 1 (BXDC1; anti-RPF2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C38
Specificity
Recognizes human Brix Domain Containing 1.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Applicable Applications for anti-RPF2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Immunocytochemistry, Dot Blot, and Western Blot.
Immunogen
Partial sequence of recombinant full-length protein to protein corresponding to amino acids located near the C-terminal region of human Brix Domain Containing 1
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis using immunogen as sample.)
Western Blot (WB)
(Western Blot analysis using immunogen as sample.)
Western Blot (WB)
(Detection of human BXDC1 by Western blot. Samples: Whole cell lysate (25ug) from HeLa cells. Predicted molecular weight: 35 kD)
Western Blot (WB)
(Detection of human BXDC1 by Western blot. Samples: Whole cell lysate (25ug) from HeLa cells. Predicted molecular weight: 35 kD)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with B758-10. Nucleus was stained with Hoechst (blue fluorescence).)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with B758-10. Nucleus was stained with Hoechst (blue fluorescence).)
Product Categories/Family for anti-RPF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
35,583 Da
NCBI Official Full Name
Brix domain containing 1
NCBI Official Synonym Full Names
ribosome production factor 2 homolog (S. cerevisiae)
NCBI Official Symbol
RPF2
NCBI Official Synonym Symbols
BXDC1; FLJ21087; bA397G5.4
NCBI Protein Information
ribosome production factor 2 homolog; homolog of Rpf2; OTTHUMP00000017009; OTTHUMP00000017010; OTTHUMP00000017011; brix domain containing 1; brix domain-containing protein 1; ribosomal processing factor 2 homolog; ribosome biogenesis protein RPF2 homolog
UniProt Protein Name
Ribosome production factor 2 homolog
Protein Family
UniProt Gene Name
RPF2
UniProt Synonym Gene Names
BXDC1
UniProt Entry Name
RPF2_HUMAN
Uniprot Description
BXDC1: Belongs to the RPF2 family.
Protein type: Nucleolus; Unknown function
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: nucleolus; nucleus
Molecular Function: rRNA binding
Biological Process: ribosomal large subunit assembly and maintenance; maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Research Articles on RPF2
Similar Products
Product Notes
The RPF2 rpf2 (Catalog #AAA602522) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Brix Domain Containing 1 (BXDC1, bA397G5.4, FLJ21087, RPF2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Brix Domain Containing 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA). Suitable for use in Immunocytochemistry, Dot Blot, and Western Blot. Researchers should empirically determine the suitability of the RPF2 rpf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Brix Domain Containing 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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