Mouse anti-Human BTK Monoclonal Antibody | anti-BTK antibody

BTK Antibody

Cat. Num. AAA9215577

• Gene Names: BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Synonyms: BTK; Monoclonal Antibody; BTK Antibody; Purified Mouse Monoclonal Antibody (Mab); Agammaglobulinaemia tyrosine kinase; AGMX 1; AGMX1; AT; ATK; B cell progenitor kinase; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton's Tyrosine Kinase; Btk; BTK_HUMAN; IMD 1; IMD1; MGC126261; MGC126262; OTTHUMP00000063593; PSCTK 1; PSCTK1; Tyrosine protein kinase BTK; Tyrosine-protein kinase BTK; XLA; anti-BTK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Form/Format: Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Sequence Length: 659

• Applicable Applications for anti-BTK antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Western Blot (WB) ~~ 1:1000; Immunoprecipitation (IP) ~~ 1:500
• Cellular location: Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note=In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in theactivation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.
• Tissue Location: Predominantly expressed in B-lymphocytes.
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of BTK in Daudi,Jurkat(BTK negative),K562,Ramos and Raji cell lysates using BTK mouse mAb (1:1000 diluted).Predicted band size:77KDa.Observed band size:77KDa.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Raji cell lysates using BTK mouse mAb.)

Related Product Information for anti-BTK antibody

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.

Product Categories/Family for anti-BTK antibody

Immunology; Signal Transduction

References

Vetrie D.,et al.Nature 361:226-233(1993). Vetrie D.,et al.Nature 364:362-362(1993). Ohta Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:9062-9066(1994). Rohrer J.,et al.Immunogenetics 40:319-324(1994). Hagemann T.L.,et al.Hum. Mol. Genet. 3:1743-1749(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 4557377
• NCBI GeneID: 695
• NCBI Accession #: NP_000052.1
• NCBI GenBank Nucleotide #: NM_000061.2
• UniProt Accession #: Q06187; Q32ML5; B2RAW1
• Molecular Weight: Calculated MW: 77kDa
• NCBI Official Full Name: tyrosine-protein kinase BTK isoform 1
• NCBI Official Synonym Full Names: Bruton agammaglobulinemia tyrosine kinase
• NCBI Official Symbol: BTK
• NCBI Official Synonym Symbols: AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• NCBI Protein Information: tyrosine-protein kinase BTK
• UniProt Protein Name: Tyrosine-protein kinase BTK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BTK
• UniProt Synonym Gene Names: AGMX1; ATK; BPK; ATK; BPK
• UniProt Entry Name: BTK_HUMAN

NCBI Description

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Uniprot Description

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; Tec family

Chromosomal Location of Human Ortholog: Xq21.33-q22

Cellular Component: extrinsic to internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; B cell activation; cell maturation; calcium-mediated signaling; T cell receptor signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; regulation of cell proliferation; toll-like receptor 2 signaling pathway; regulation of B cell cytokine production; regulation of B cell apoptosis; MyD88-dependent toll-like receptor signaling pathway; B cell receptor signaling pathway; negative regulation of cytokine production; toll-like receptor signaling pathway; innate immune response; positive regulation of B cell differentiation; mesoderm development; cell differentiation; toll-like receptor 4 signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Agammaglobulinemia, X-linked; Isolated Growth Hormone Deficiency, Type Iii