ELISA (EIA)
(ELISA of anti-BSA antibody on BSA. Binding curves of the rabbit IgG chimeric version of the anti-BSA antibody D1 (; blue line) and isotype control (MBS488041, anti-fluorescein; orange line) to an ELISA plate coated with BSA at a concentration of 5ug/ml. A 3-fold serial dilution from 90ug/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an HRP-conjugated anti-rabbit IgG antibody was used.)
Rabbit anti-Bovine BSA Monoclonal Antibody | anti-BSA antibody
Anti-BSA [D1]
Reactivity
Bovine
Applications
Immunoprecipitation
Purity
Purified antibody. Protein A affinity purified
Synonyms
BSA; Monoclonal Antibody; Anti-BSA [D1]; Bovine serum albumin; serum albumin; albumin; anti-BSA antibody
Host
Rabbit
Reactivity
Bovine
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
D1
Specificity
D1 has been shown to bind to BSA in panning experiments from phage display. Albumin is a secreted globular protein found in blood plasma and BSA has multiple biochemical applications including ELISAs and immunohistochemistry and is able to increase the stability of proteins and be used as a standard in Bradford assays.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BSA antibody
Immunoprecipitation (IP)
Application Notes
D1 was selected for by panning where it reacted postiively on BSA. D1 can also be used as a control in IP and when working with native proteins when conducting studies within a BSA medium.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
PMID
9729530
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
ELISA (EIA)
(ELISA of anti-BSA antibody on BSA. Binding curves of the rabbit IgG chimeric version of the anti-BSA antibody D1 (; blue line) and isotype control (MBS488041, anti-fluorescein; orange line) to an ELISA plate coated with BSA at a concentration of 5ug/ml. A 3-fold serial dilution from 90ug/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an HRP-conjugated anti-rabbit IgG antibody was used.)
ELISA (EIA)
(ELISA of anti-BSA antibody on BSA. Binding curves of the rabbit IgG chimeric version of the anti-BSA antibody D1 (; blue line) and isotype control (MBS488041, anti-fluorescein; orange line) to an ELISA plate coated with BSA at a concentration of 5ug/ml. A 3-fold serial dilution from 90ug/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an HRP-conjugated anti-rabbit IgG antibody was used.)
Related Product Information for anti-BSA antibody
Recombinant monoclonal antibody to BSA. Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody D1.
D1 was prepared by isolating anti-p24 scFvs from a phage display library made from a mouse immunized with lysate from HIV-1 infected H9 cells.
D1 was prepared by isolating anti-p24 scFvs from a phage display library made from a mouse immunized with lysate from HIV-1 infected H9 cells.
Product Categories/Family for anti-BSA antibody
References
de Haard et al. Selection of recombinant, library-derived antibody fragments against p24 for human immunodeficiency virus type 1 diagnostics. Clin Diagn Lab Immunol. 1998 Sep;5(5):636-44.
Note on publication: Describes the production of the anti-BSA antibody D1 in addition to antibodies specific for the viral protein p24.
Note on publication: Describes the production of the anti-BSA antibody D1 in addition to antibodies specific for the viral protein p24.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
160,000
NCBI Official Full Name
serum albumin
NCBI Official Synonym Full Names
albumin
NCBI Official Symbol
ALB
NCBI Protein Information
serum albumin; BSA
UniProt Protein Name
Serum albumin
UniProt Gene Name
ALB
UniProt Entry Name
ALBU_BOVIN
Uniprot Description
albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc
Protein type: Secreted, signal peptide; Secreted; Carrier
Cellular Component: protein complex; cytoplasm; extracellular region; nucleus
Molecular Function: toxin binding; DNA binding; metal ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding
Biological Process: retinal homeostasis; maintenance of mitochondrion localization; transport; hemolysis by symbiont of host red blood cells; cellular response to starvation; negative regulation of apoptosis
Research Articles on BSA
Similar Products
Product Notes
The BSA alb (Catalog #AAA488576) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BSA [D1] reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's BSA can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). D1 was selected for by panning where it reacted postiively on BSA. D1 can also be used as a control in IP and when working with native proteins when conducting studies within a BSA medium. Researchers should empirically determine the suitability of the BSA alb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BSA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
