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Immunohistochemistry - Paraffin (IHC) (Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE))

Mouse anti-Human BPIFB1 Monoclonal Antibody | anti-BPIFB1 antibody

Anti-BPIFB1 Antibody (clone 2A5) IHC-plus

Gene Names
BPIFB1; LPLUNC1; C20orf114
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Purified
Synonyms
BPIFB1; Monoclonal Antibody; Anti-BPIFB1 Antibody (clone 2A5) IHC-plus; Mouse Monoclonal [clone 2A5] (IgG1) to Human BPIFB1; Human BPIFB1; C20orf114; DJ1187J4.1; BA49G10.6; VEMSGP; LPLUNC1; anti-BPIFB1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2A5
Specificity
Human BPIFB1
Purity/Purification
Purified
Form/Format
PBS, 0.05% sodium azide, 0.05% protein stabilizer
Concentration
1 mg/ml (varies by lot)
Sequence Length
484
Applicable Applications for anti-BPIFB1 antibody
Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
ELISA (1:10000)
Flow Cytometry (1:200 - 1:400)
ICC (1:200 - 1:1000)
IF (1:1000)
IHC-P (10 ug/ml)
WB (1:500 - 1:2000)
Immunogen
BPIFB1 antibody was raised against purified recombinant fragment of human LPlunc1 expressed in E Coli.
Immunogen Description
Purified recombinant fragment of human LPlunc1 expressed in E Coli.
Immunogen Type
Recombinant protein
Target Species
Human
Preparation and Storage
Long term: -20 degree C
Short term: 4 degree C
Avoid freeze-thaw cycles.

Immunohistochemistry - Paraffin (IHC)

(Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry - Paraffin (IHC) (Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescence of HeLa cells using LPlunc1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

Immunofluorescence (IF) (Immunofluorescence of HeLa cells using LPlunc1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

Western Blot (WB)

(Western blot using LPlunc1 monoclonal antibody against human LPlunc1 recombinant protein. (Expected MW is 52 kDa))

Western Blot (WB) (Western blot using LPlunc1 monoclonal antibody against human LPlunc1 recombinant protein. (Expected MW is 52 kDa))

ELISA (EIA)

(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)

ELISA (EIA) (Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
Related Product Information for anti-BPIFB1 antibody
BPIFB1 Antibody, C20orf114 Antibody, DJ1187J4.1 Antibody, BA49G10.6 Antibody, VEMSGP Antibody, LPLUNC1 Antibody Description: May play a role in innate immunity in mouth, nose and lungs. Binds bacterial lipopolysaccharide (LPS) and modulates the cellular responses to LPS.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,695 Da
NCBI Official Full Name
BPI fold-containing family B member 1
NCBI Official Synonym Full Names
BPI fold containing family B member 1
NCBI Official Symbol
BPIFB1
NCBI Official Synonym Symbols
LPLUNC1; C20orf114
NCBI Protein Information
BPI fold-containing family B member 1
UniProt Protein Name
BPI fold-containing family B member 1
UniProt Gene Name
BPIFB1
UniProt Synonym Gene Names
C20orf114; LPLUNC1; VEMSGP
UniProt Entry Name
BPIB1_HUMAN

NCBI Description

The protein encoded by this gene may be involved in the innate immune response to bacterial exposure in the mouth, nasal cavities, and lungs. The encoded protein is secreted and is a member of the BPI/LBP/PLUNC protein superfamily. This gene is found with other members of the superfamily in a cluster on chromosome 20. [provided by RefSeq, Jul 2008]

Uniprot Description

LPLUNC1: May play a role in innate immunity in mouth, nose and lungs. Belongs to the BPI/LBP/Plunc superfamily. Plunc family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: extracellular region; extracellular space

Molecular Function: lipid binding

Biological Process: innate immune response in mucosa; negative regulation of toll-like receptor 4 signaling pathway

Research Articles on BPIFB1

Similar Products

Product Notes

The BPIFB1 bpifb1 (Catalog #AAA249438) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-BPIFB1 Antibody (clone 2A5) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BPIFB1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA). ELISA (1:10000) Flow Cytometry (1:200 - 1:400) ICC (1:200 - 1:1000) IF (1:1000) IHC-P (10 ug/ml) WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the BPIFB1 bpifb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BPIFB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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