Mouse anti-Human BPI Monoclonal Antibody | anti-BPI antibody

BPI, Human, mAb 3F9

Cat. Num. AAA584163

• Gene Names: BPI; rBPI; BPIFD1
• Reactivity: Human
• Applications: Immunoassay (IA)
• Synonyms: BPI; Monoclonal Antibody; Human; mAb 3F9; anti-BPI antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 487

• Applicable Applications for anti-BPI antibody: Immunoassay (IA)
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-BPI antibody

Product Description: The monoclonal antibody 3F9 reacts specifically with full length human natural and recombinant Bactericidal Permeability Increasing protein (BPI). The antimicrobial protein BPI is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. BPI is a bactericidal compound that is present in polymorphonuclear cells (PMN) and in lower levels in the specific granules of eosinophils. BPI possesses high affinity toward the lipid A region of lipopolysaccharides (LPS) that comprise the outer leaflet of the gram-negative bacterial outer membrane. Binding of BPI to the lipid A moiety of LPS exerts multiple anti-infective activities against gram-negative bacteria: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. Airway epithelial cells constitutively express the BPI gene and produce the BPI protein and, therefore, BPI may be a critical determinant in the development of LPS-triggered airways disease. Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation. Furthermore, a 21 kDa bioactive recombinant fragment of BPI, rBPI21, was shown to confer a survival advantage against invasive pneumococcal disease by binding to the gram-positive bacterial pathogen, pneumolysin. The monoclonal antibody 3F9 recognizes only free BPI and does not interact with BPI that has formed a complex with LPS.

NCBI and Uniprot Product Information

• NCBI GI #: 157276599
• NCBI GeneID: 671
• NCBI Accession #: NP_001716.2
• NCBI GenBank Nucleotide #: NM_001725.2
• Molecular Weight: 53,900 Da
• NCBI Official Full Name: bactericidal permeability-increasing protein
• NCBI Official Synonym Full Names: bactericidal/permeability-increasing protein
• NCBI Official Symbol: BPI
• NCBI Official Synonym Symbols: rBPI; BPIFD1
• NCBI Protein Information: bactericidal permeability-increasing protein; CAP 57; recombinant BPI holoprotein, rBPI; BPI fold containing family D, member 1
• UniProt Protein Name: Bactericidal permeability-increasing protein
• Protein Family: BPI fold-containing family
• UniProt Gene Name: BPI
• UniProt Synonym Gene Names: BPI
• UniProt Entry Name: BPI_HUMAN

NCBI Description

This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has bactericidal activity on gram-negative organisms. [provided by RefSeq, Jul 2008]

Uniprot Description

BPI: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein type: Secreted, signal peptide; Cell surface; Secreted

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: integral to plasma membrane; cytoplasm

Molecular Function: lipopolysaccharide binding

Biological Process: negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; immune response; negative regulation of tumor necrosis factor production; negative regulation of macrophage activation; defense response to Gram-negative bacterium